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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >A Predominant and Virulent Legionella pneumophila Serogroup 1 Strain Detected in Isolates from Patients and Water in Queensland Australia by an Amplified Fragment Length Polymorphism Protocol and Virulence Gene-Based PCR Assays
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A Predominant and Virulent Legionella pneumophila Serogroup 1 Strain Detected in Isolates from Patients and Water in Queensland Australia by an Amplified Fragment Length Polymorphism Protocol and Virulence Gene-Based PCR Assays

机译：通过扩增的片段长度多态性协议和基于毒力基因的PCR检测方法在澳大利亚昆士兰州的患者和水中分离出的一种优势菌和强毒嗜肺军团菌血清群1菌株

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摘要

In epidemiological investigations of community legionellosis outbreaks, knowledge of the prevalence, distribution, and clinical significance (virulence) of environmental Legionella isolates is crucial for interpretation of the molecular subtyping results. To obtain such information for Legionella pneumophila serogroup 1 isolates, we used the standardized amplified fragment length polymorphism (AFLP) protocol of the European Working Group on Legionella Infection to subtype L. pneumophila SG1 isolates obtained from patients and water sources in Queensland, Australia. An AFLP genotype, termed AF1, was predominant in isolates from both patients (40.5%) and water (49.0%). The second most common AFLP genotype found in water isolates was AF16 (36.5%), but this genotype was not identified in the patient isolates. When virulence gene-based PCR assays for lvh and rtxA genes were applied to the isolates from patients and water, nearly all (65 of 66) AF1 strains had both virulence genes, lvh and rtxA. In contrast, neither the lvh nor the rtxA gene was found in the AF16 strains, except for one isolate with the rtxA gene. It appears that this may explain the failure to find this genotype in the isolates from patients even though it may be common in the environment. In view of the evidence that the AF1 genotype is the most common genotype among strains found in patients and water sources in this region, any suggested epidemiological link derived from comparing the AF1 genotype from patient isolates with the AF1 genotype from environmental isolates must be interpreted and acted on with caution. The use of virulence gene-based PCR assays applied to environmental samples may be helpful in determining the infection potential of the isolates involved.
机译：在社区军团菌病暴发的流行病学调查中，环境军团菌分离株的流行，分布和临床意义（毒力）的知识对于解释分子亚型分析结果至关重要。为了获得嗜肺军团菌血清群1分离株的此类信息，我们使用了欧洲军团菌感染工作组针对从澳大利亚昆士兰州的患者和水源获得的嗜肺军团杆菌SG1亚型的标准化扩增片段长度多态性（AFLP）方案。在来自患者（40.5％）和水（49.0％）的分离物中，AFLP基因型称为AF1。在水分离株中发现的第二个最常见的AFLP基因型是AF16（36.5％），但在患者分离株中未发现该基因型。当将基于lvh和rtxA基因的基于毒力基因的PCR分析应用于患者和水中的分离株时，几乎所有（66个中的65个）AF1菌株都具有lvh和rtxA毒力基因。相反，除了一种带有rtxA基因的分离株外，在AF16菌株中均未发现lvh和rtxA基因。似乎这可以解释未能从患者的分离物中找到该基因型的原因，即使在环境中可能很常见。鉴于有证据表明AF1基因型是该地区患者和水源中最常见的菌株，因此，应解释和比较将患者分离株的AF1基因型与环境分离株的AF1基因型进行比较得出的任何流行病学联系。谨慎行事。在环境样品中使用基于毒力基因的PCR分析可能有助于确定相关菌株的感染潜力。

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