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首页> 美国卫生研究院文献>Genes >Comparative Transcriptome Analysis Identifying the Different Molecular Genetic Markers Related to Production Performance and Meat Quality in Longissimus Dorsi Tissues of MG × STH and STH Sheep
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Comparative Transcriptome Analysis Identifying the Different Molecular Genetic Markers Related to Production Performance and Meat Quality in Longissimus Dorsi Tissues of MG × STH and STH Sheep

机译：比较转录组分析鉴定MG×STH和STH绵羊背最长背组织生产性能和肉品质相关的不同分子遗传标记

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摘要

Crossbred sheep have many prominent traits, such as excellent production performance and high-quality meat, when compared to local sheep breeds. However, the genetic molecular markers related to these characteristics remain unclear. The crossbred MG × STH (small-tailed Han sheep (STH) × Mongolian sheep (MG)) breed and the STH breed were selected to measure production performance and meat quality. We used 14 indexes of production performance and meat quality, which in the MG × STH population showed significant differences compared to the STH breed. Subsequently, the from the two sheep were subjected to comparative transcriptomic analyses to identify differentially expressed genes (DEGs) related to production performance and meat quality. A total of 874 DEGs were identified between the two sheep groups. A total of 110 unique DEGs related to sheep production performance and meat quality were selected as the candidate DEGs. We found 6 production-performance-related and 30 meat-quality-related DEGs through a correlation analysis, including , , , and . The expression levels of 11 DEGs were validated by real-time PCR, and the results were in accordance with the results of the comparative transcriptomic and correlation analyses. These results will assist in understanding sheep heterosis and molecular marker-assisted selection.
机译：与当地绵羊品种相比，杂交绵羊具有许多突出的特征，例如出色的生产性能和高质量的肉。然而，与这些特征有关的遗传分子标记仍然不清楚。选择杂交MG×STH（小尾寒羊（STH）×蒙古羊（MG））品种和STH品种来测量生产性能和肉质。我们使用了14个生产性能和肉质指标，在MG×STH种群中，与STH品种相比有显着差异。随后，对这两只绵羊的绵羊进行了比较转录组学分析，以鉴定与生产性能和肉质相关的差异表达基因（DEG）。在两个绵羊群之间总共鉴定出874个DEG。总共选择了与绵羊生产性能和肉质相关的110个独特DEG作为候选DEG。通过相关分析，包括，，和，我们发现了6个与生产性能相关的DEG和30个与肉质相关的DEG。通过实时PCR验证了11个DEGs的表达水平，其结果与比较转录组学和相关分析的结果相符。这些结果将有助于理解绵羊杂种优势和分子标记辅助选择。

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