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Ovarian Transcriptomic Analyses in the Urban Human Health Pest the Western Black Widow Spider

机译:西方人类黑寡妇蜘蛛对城市人类健康害虫的卵巢转录组学分析

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摘要

Due to their abundance and ability to invade diverse environments, many arthropods have become pests of economic and health concern, especially in urban areas. Transcriptomic analyses of arthropod ovaries have provided insight into life history variation and fecundity, yet there are few studies in spiders despite their diversity within arthropods. Here, we generated a ovarian transcriptome from 10 individuals of the western black widow spider ( ), a human health pest of high abundance in urban areas, to conduct comparative ovarian transcriptomic analyses. Biological processes enriched for metabolism—specifically purine, and thiamine metabolic pathways linked to oocyte development—were significantly abundant in . Functional and pathway annotations revealed overlap among diverse arachnid ovarian transcriptomes for highly-conserved genes and those linked to fecundity, such as oocyte maturation in vitellogenin and vitelline membrane outer layer proteins, hormones, and hormone receptors required for ovary development, and regulation of fertility-related genes. Comparative studies across arachnids are greatly needed to understand the evolutionary similarities of the spider ovary, and here, the identification of ovarian proteins in provides potential for understanding how increased fecundity is linked to the success of this urban pest.
机译:由于其节肢动物的丰富和入侵各种环境的能力,许多节肢动物已成为经济和健康问题的害虫,特别是在城市地区。对节肢动物卵巢的转录组学分析提供了关于生活史变异和繁殖力的见解,尽管在节肢动物中它们具有多样性,但对蜘蛛的研究很少。在这里,我们从10个西方黑寡妇蜘蛛()(一个城市地区人口丰富的人类健康害虫)的个体中产生了一个卵巢转录组,以进行比较性的卵巢转录组分析。富含代谢的生物过程-特别是嘌呤和与卵母细胞发育有关的硫胺素代谢途径-在该国中非常丰富。功能和途径注释揭示了高度保守基因的各种蛛网膜卵巢转录组与与生殖力相关的转录组重叠,例如卵黄蛋白原和卵黄质膜外层蛋白,卵母细胞和卵子发育所需的卵母细胞成熟,卵巢发育所需的激素以及对生育力的调节相关基因。为了了解蜘蛛卵巢的进化相似性,非常需要跨蜘蛛的比较研究,在这里,卵巢蛋白的鉴定提供了潜在的潜力,以了解繁殖力如何与这种城市害虫的成功联系在一起。

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