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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei: a Novel Approach Using Lineage-Specific Genes
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MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei: a Novel Approach Using Lineage-Specific Genes

机译：基于MP1同源性的多位点序列系统用于键入病原性真菌马尔尼菲青霉：一种使用谱系特异性基因的新方法

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A highly reproducible and discriminative typing system is essential for better understanding of the epidemiology of Penicillium marneffei, the most important thermal dimorphic fungus causing respiratory, skin, and systemic mycosis in Southeast Asia. The sequences of 11 housekeeping genes were identical among 10 strains of P. marneffei, but those of MP1 and its 13 homologues, a novel superfamily of mannoproteins in the subdivision Pezizomycotina of Ascomycetes, mostly species of Penicillium and Aspergillus, showed significant variations. Therefore, a multilocus sequence typing (MLST) system for P. marneffei was constructed using MP1 (549 bp) and the four of its homologues (MPLP4 [337 bp], MPLP7 [347 bp], MPLP10 [546 bp], and MPLP13 [422 bp]) that showed the greatest variations. Among the 2,201 bp of the five loci, 183 polymorphic sites were observed in 44 strains of P. marneffei. The median number of alleles at each locus was five (range, 5 [MPLP4, MPLP7, and MPLP13] to 15 [MPLP10]). Four of the five genes had nonsynonymous substitution/synonymous substitution (dn/ds) ratios of >1. A total of 35 different sequence types (STs) were assigned to the 44 P. marneffei isolates, with 28 of the 35 STs identified only once. The discriminatory power was 0.9884. MP1 and its homologues were better than housekeeping genes for MLST in P. marneffei. Due to their more rapid evolutionary rates, lineage-specific genes may be better candidates than housekeeping genes for sequence-based typing, especially in microbes that evolve slowly or have evolved recently.
机译：为了更好地了解马尔尼菲青霉（Penicillium marneffei）的流行病学，高度可重复和有区别的分型系统是必不可少的，马尔尼菲青霉是引起东南亚呼吸，皮肤和系统性真菌病的最重要的热二形真菌。在10个Marneffei菌株中，11个管家基因的序列相同，但MP1及其13个同系物是在子囊菌Pezizomycotina细分中的一种新的甘露糖蛋白超家族，主要是青霉和Aspergillus。因此，使用MP1（549 bp）及其四个同源物（MPLP4 [337 bp]，MPLP7 [347 bp]，MPLP10 [546 bp]和MPLP13 [ 422 bp]）。在5个基因座的2,201 bp中，在44个马尔涅菲疟原虫菌株中观察到183个多态性位点。每个位点的等位基因中位数为5个（范围：5 [MPLP4，MPLP7和MPLP13]至15 [MPLP10]）。这五个基因中有四个的非同义替换/同义替换（ dn / ds ）比> 1。总共35种不同的序列类型（ST）被分配给了44个 P。 marneffei 分离株，在35个ST中只有28个被鉴定出一次。判别力为0.9884。在 P中， MP1 及其同系物比MLST的管家基因更好。 marneffei 。由于其更快的进化速度，谱系特异性基因可能比管家基因更适合用于基于序列的分型，特别是在进化缓慢或最近进化的微生物中。

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