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首页> 美国卫生研究院文献>Heliyon >Chemokine cytokine and haematological profiles in Sprague-Dawley rats co-infected with Plasmodium berghei ANKA and Trichinella zimbabwensis-A laboratory animal model for malaria and tissue-dwelling nematodes co-infection
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Chemokine cytokine and haematological profiles in Sprague-Dawley rats co-infected with Plasmodium berghei ANKA and Trichinella zimbabwensis-A laboratory animal model for malaria and tissue-dwelling nematodes co-infection

机译：伯氏疟原虫ANKA和津巴布韦旋毛虫共感染的Sprague-Dawley大鼠的趋化因子细胞因子和血液学特征-疟疾和组织线虫共感染的实验动物模型

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Malaria remains a major cause of mortality and morbidity in sub-Saharan Africa (SSA) and tissue-dwelling helminth parasites (TDHPs) are also prevalent in this region presenting a geographical overlap in endemicity. There is paucity of information on the specific host immune responses elicited at different phases of the life cycle by the co-infecting helminth parasites. This study aimed at using a laboratory animal model to determine selected chemokine, cytokine and hematological profiles in Sprague-Dawley rats co-infected with ANKA (Pb) and a tissue-dwelling nematode, (Tz). One-hundred-and-sixty-eight male Sprague-Dawley rats (90–150g) were randomly divided into four experimental groups; Control (n = 42), Pb-infected (n = 42), Tz-infected (n = 42) and Pb Tz-infected group (n = 42). infection (3 muscle larvae/g body weight ) was done on day 0 while intra-peritoneal Pb infection (10 parasitised RBCs) was done at day 28 of the 42-day experimental study for the co-infection group which corresponded with day 0 of the Pb group on the protocol. Haematological parameters, cytokines (TNF-α, IL-10, IL-4, IL-6), chemokines (CXCL10, CCL5, CCL11) and burden of Tz adult worms and muscle larvae burden were determined as per need for each group. Results showed that Tz infection predisposed the co-infected animals towards rapid development of Pb parasitaemia during co-infection, reaching a higher peak percentage parasitaemia at day 7 post-infection than the Pb mono-infected group at day 6 post-infection. Animals in the co-infected group also exhibited severe anaemia, basophilia, neutrophilia, eosinophilia and lymphopenia at day 7 post Pb infection compared to the control groups. Significant elevation of Pb parasitaemia coincided with elevated pro-inflammatory cytokine TNF-α ( 0.001), regulatory anti-inflammatory IL-10 ( 0.001), and pro-inflammatory chemokines CXCL10 ( 0.001) concentration in comparison to control group, at day 7 post Pb infection. Our results confirm that co-infection of Pb with Tz resulted in increased Pb parasitaemia compared to the control group in the early stages of infection and this might translate to severe malaria.
机译：疟疾仍然是撒哈拉以南非洲（SSA）死亡率和发病率的主要原因，并且在该地区还普遍存在居住于组织中的蠕虫寄生虫（TDHP），在地方性流行方面存在地理重叠。共同感染蠕虫寄生虫在生命周期的不同阶段引发的特定宿主免疫反应的信息很少。这项研究旨在使用实验室动物模型来确定与ANKA（Pb）和组织居住线虫（Tz）共感染的Sprague-Dawley大鼠的选定趋化因子，细胞因子和血液学特征。 168只雄性Sprague-Dawley大鼠（90-150g）被随机分为四个实验组。对照组（n = 42），Pb感染（n = 42），Tz感染（n = 42）和Pb Tz感染组（n = 42）。共感染组在42天实验研究中，在第0天进行了感染（3例肌肉幼虫/克体重），而在第28天进行了腹膜内Pb感染（10例寄生红细胞），对应于第0天。协议上的Pb组。根据每组的需要确定血液学参数，细胞因子（TNF-α，IL-10，IL-4，IL-6），趋化因子（CXCL10，CCL5，CCL11）以及Tz成虫的蠕虫负担和肌肉幼虫的负担。结果表明，Tz感染使共同感染的动物在共同感染期间容易出现Pb寄生虫血症，在感染后第7天达到的峰值寄生虫百分率高于在感染后第6天感染Pb单一感染的人群。与对照组相比，共感染组中的动物在Pb感染后第7天还表现出严重的贫血，嗜碱性粒细胞，嗜中性粒细胞，嗜酸性粒细胞增多和淋巴细胞减少。与对照组相比，在术后第7天，Pb寄生虫血症的显着升高与促炎细胞因子TNF-α（0.001），调节性抗炎IL-10（0.001）和促炎性趋化因子CXCL10（0.001）升高同时发生铅感染。我们的结果证实，在感染的早期，与对照组相比，铅与Tz的共同感染导致铅寄生虫血症增加，这可能转化为严重的疟疾。

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