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Comparison of conventional extraction technique with ultrasound assisted extraction on recovery of phenolic compounds from lemon scented tea tree (Leptospermum petersonii) leaves

机译:传统提取技术与超声辅助提取对柠檬香茶树(Leptospermum petersonii)叶片中酚类化合物回收的比较

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摘要

is a native Australian medicinal and aromatic plant. This study was designed to evaluate the influence of solvents and ultrasound-assisted extraction (UAE) parameters including time, temperature, and sonication power on the yield of phenolic compounds and antioxidant capacity from lemon scented tea tree leaves. Extraction efficiency of the optimal UAE conditions were compared with that of shaking water bath technique. The results show that extraction solvents significantly affect extraction yield of phenolic compounds and antioxidant properties, and 50% acetone in water was found to be the most suitable solvent. The UAE optimal conditions were 60 min, 50 °C and sonication power of 200 W. Under these optimal conditions the yields of total phenolics, flavonoids, proanthocyanidins were 98.91 ± 1.20 (mg GAE/g DW), 76.12 ± 0.79 (mg CE/g DW), 117.71 ± 2.18 (mg CE/g DW), respectively. Antioxidant properties from four assays including FRAP, CUPRAC, ABTS and DPPH were 581.29 ± 14.23, 5534.87 ± 19.56, 1636.18 ± 4.11, and 889.29 ± 20.68 (mM TE/g DW) respectively. The UAE extraction technique was found to be more efficient in extraction of total phenolics and antioxidant capacity in comparison with conventional shaking water bath extraction. This study also observed a strong correlation between phenolic compounds and antioxidant capacities. All three phenolic compound groups (TPC, TFC, and Pro.A) were contributed to both free radical scavenging and ion reducing properties in the lemon scented tea tree leaves extract. However, the order of the phenolic groups was TPC > Pro.A > TFC for antioxidant properties.
机译:是澳大利亚本土的药用和芳香植物。本研究旨在评估溶剂和超声辅助提取(UAE)参数(包括时间,温度和超声处理能力)对柠檬香茶树叶片中酚类化合物的产量和抗氧化能力的影响。将最佳阿联酋条件的提取效率与摇动水浴技术的提取效率进行了比较。结果表明,萃取溶剂显着影响酚类化合物的萃取率和抗氧化性能,发现50%的丙酮在水中是最合适的溶剂。阿联酋的最佳条件为60分钟,50°C,超声功率为200W。在这些最佳条件下,总酚,类黄酮,原花色素的产率为98.91±1.20(mg GAE / g DW),76.12±0.79(mg CE / g DW),117.71±2.18(mg CE / g DW)。来自FRAP,CUPRAC,ABTS和DPPH四种测定的抗氧化特性分别为581.29±14.23、5534.87±19.56、1636.18±4.11和889.29±20.68(mM TE / g DW)。与传统的摇摇水浴萃取相比,阿联酋萃取技术被发现在总酚类和抗氧化能力的萃取上更有效。这项研究还观察到酚类化合物与抗氧化能力之间存在很强的相关性。所有三个酚类化合物基团(TPC,TFC和Pro.A)都有助于柠檬香茶树叶提取物中的自由基清除和离子还原性能。但是,酚基的抗氧化性能顺序为:TPC> Pro.A> TFC。

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