• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Insects >The ABCB Multidrug Resistance Proteins Do Not Contribute to Ivermectin Detoxification in the Colorado Potato Beetle Leptinotarsa decemlineata (Say)
【2h】

The ABCB Multidrug Resistance Proteins Do Not Contribute to Ivermectin Detoxification in the Colorado Potato Beetle Leptinotarsa decemlineata (Say)

机译：ABCB多药抗性蛋白对科罗拉多马铃薯甲虫Leptinotarsa decemlineata中的伊维菌素解毒没有贡献（说）

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Colorado potato beetle, (Say), is a significant agricultural pest that has developed resistance to many insecticides that are used to control it. Investigating the mechanisms of insecticide detoxification in this pest is important for ensuring its continued control, since they may be contributors to such resistance. Multidrug resistance (MDR) genes that code for the ABCB transmembrane efflux transporters are one potential source of insecticide detoxification activity that have not been thoroughly examined in . In this study, we annotated the ABCB genes found in the genome and then characterized the expression profiles across midgut, nerve, and Malpighian tubule tissues of the three full transporters identified. To investigate if these genes are involved in defense against the macrocyclic lactone insecticide ivermectin in this insect, each gene was silenced using RNA interference or MDR protein activity was inhibited using a chemical inhibitor, verapamil, before challenging the insects with a dose of ivermectin. Survival of the insects did not significantly change due to gene silencing or protein inhibition, suggesting that MDR transporters do not significantly contribute to defense against ivermectin in .
机译：科罗拉多马铃薯甲虫（Say）是一种重要的农业害虫，已对用于控制它的许多杀虫剂产生了抗性。调查该害虫中的杀虫剂解毒机理对于确保其持续控制很重要，因为它们可能是这种抗药性的原因。编码ABCB跨膜外排转运蛋白的多药抗性（MDR）基因是杀虫剂解毒活性的一种潜在来源，尚未在中进行彻底检查。在这项研究中，我们注释了在基因组中发现的ABCB基因，然后表征了鉴定出的三个完整转运蛋白在中肠，神经和Malpighian小管组织中的表达谱。为了研究这些基因是否参与了该昆虫对大环内酯杀虫剂伊维菌素的防御，在使用一定剂量的伊维菌素对昆虫进行攻击之前，使用RNA干扰使每个基因沉默，或使用化学抑制剂维拉帕米抑制MDR蛋白活性。由于基因沉默或蛋白质抑制，昆虫的存活率没有显着变化，这表明MDR转运蛋白在防御伊维菌素中没有显着贡献。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号