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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae)
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Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae)

机译：板栗种子（菊科）淀粉代谢相关基因的转录组分析与鉴定

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摘要

Starch is the most important form of carbohydrate storage and is the major energy reserve in some seeds, especially . Seed germination is the beginning of the plant’s life cycle, and starch metabolism is important for seed germination. As a complex metabolic pathway, the regulation of starch metabolism in is still poorly understood. To explore the mechanism of starch metabolism during the germination of , we conducted a comparative gene expression analysis at the transcriptional level using RNA-seq across four different germination stages, and analyzed the changes in the starch and soluble sugar contents. The results showed that the starch content increased in 0–10 days and decreased in 10–35 days, while the soluble sugar content continuously decreased in 0–30 days and increased in 30–35 days. We identified 49 candidate genes that may be associated with starch and sucrose metabolism. Three ADP-glucose pyrophosphorylase (AGPase) genes, two nucleotide pyrophosphatase/phosphodiesterases (NPPS) genes and three starch synthases (SS) genes may be related to starch accumulation. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression levels of these genes. Our study combined transcriptome data with physiological and biochemical data, revealing potential candidate genes that affect starch metabolism during seed germination, and provides important data about starch metabolism and seed germination in seed plants.
机译：淀粉是碳水化合物储存的最重要形式，也是某些种子尤其是主要的能量储备。种子发芽是植物生命周期的开始，淀粉代谢对于种子发芽很重要。作为复杂的代谢途径，淀粉代谢的调控仍知之甚少。为了探索淀粉发芽过程中淀粉代谢的机制，我们在四个不同的发芽阶段使用RNA-seq在转录水平上进行了比较基因表达分析，并分析了淀粉和可溶性糖含量的变化。结果表明，淀粉含量在0–10天增加，在10–35天减少，而可溶性糖含量在0–30天连续下降，并在30–35天增加。我们确定了可能与淀粉和蔗糖代谢有关的49个候选基因。三个ADP-葡萄糖焦磷酸化酶（AGPase）基因，两个核苷酸焦磷酸酶/磷酸二酯酶（NPPS）基因和三个淀粉合酶（SS）基因可能与淀粉积累有关。实时定量聚合酶链反应（qRT-PCR）用于验证这些基因的表达水平。我们的研究结合了转录组数据和生理生化数据，揭示了可能影响种子萌发过程中淀粉代谢的候选基因，并提供了有关种子植物中淀粉代谢和种子萌发的重要数据。

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