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The Liposome PCR Assay Is More Sensitive than the Vibrio cholerae Enterotoxin and Escherichia coli Heat-Labile Enterotoxin Reversed Passive Latex Agglutination Test at Detecting Cholera Toxin in Feces and Water

机译：脂质体PCR检测比霍乱弧菌肠毒素和大肠杆菌不耐热肠毒素反向被动胶凝集试验更灵敏可检测粪便和水中的霍乱毒素

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摘要

Practical detection of cholera toxin (CT) by a liposome PCR (LPCR) immunoassay was compared to that of an established V. cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination (VET-RPLA) assay. LPCR detected CT in the range of 10 pg/ml to 100 ng/ml in simulated feces and environmental water. Detection by VET-RPLA required at least 4 to 19 ng/ml CT.

机译：比较了通过脂质体PCR（LPCR）免疫测定法对霍乱毒素（CT）的实际检测与已建立的霍乱弧菌肠毒素和大肠杆菌的热不稳定肠毒素反向被动胶乳凝集（VET-RPLA）测定的比较。 LPCR在模拟粪便和环境水中检测到的CT范围为10 pg / ml至100 ng / ml。通过VET-RPLA检测至少需要4到19 ng / ml的CT。

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期刊名称 Journal of Clinical Microbiology
作者
David L. Evers; Junkun He; Jeffrey T. Mason; Timothy J. OLeary;
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作者单位

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年(卷),期 2010(48),12
年度 2010
页码 4620–4622
总页数 3
原文格式 PDF
正文语种
中图分类微生物学;
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1. The Liposome PCR Assay Is More Sensitive than the Vibrio cholerae Enterotoxin and Escherichia coli Heat-Labile Enterotoxin Reversed Passive Latex Agglutination Test at Detecting Cholera Toxin in Feces and Water [J] . David L. Evers, Junkun He, Jeffrey T. Mason, Journal of Clinical Microbiology . 2010,第12期

机译：脂质体PCR检测比霍乱弧菌肠毒素和大肠杆菌不耐热肠毒素反向被动胶凝集试验更灵敏，可检测粪便和水中的霍乱毒素
2. Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli. [J] . T Ito, S Kuwahara, T Yokota Journal of Clinical Microbiology . 1983,第1期

机译：自动和手动乳胶凝集测试，用于测量大肠杆菌的霍乱毒素和不耐热肠毒素。
3. Specificity of the Type II Secretion Systems of Enterotoxigenic Escherichia coli and Vibrio cholerae for Heat-Labile Enterotoxin and Cholera Toxin [J] . Benjamin Mudrak, Meta J. Kuehn Journal of bacteriology . 2010,第7期

机译：产肠毒素的大肠杆菌和霍乱弧菌II型分泌系统对不耐热肠毒素和霍乱毒素的特异性
4. Integrating structure-based drug design and multivalency for creating effective antagonists of cholera toxin and Escherichia coli heat-labile enterotoxin. [D] . Pickens, Jason C. 2003

机译：整合基于结构的药物设计和多价药物，以创建有效的霍乱毒素和大肠杆菌热不稳定肠毒素拮抗剂。
5. Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli. [O] . T Ito, S Kuwahara, T Yokota 1983

机译：自动和手动乳胶凝集测试用于测量大肠杆菌的霍乱毒素和不耐热肠毒素。
6. The Liposome PCR Assay Is More Sensitive than the Vibrio cholerae Enterotoxin and Escherichia coli Heat-Labile Enterotoxin Reversed Passive Latex Agglutination Test at Detecting Cholera Toxin in Feces and Water ▿ [O] . Evers, David L., He, Junkun, Mason, Jeffrey T., 2010

机译：脂质体PCR检测比霍乱弧菌肠毒素和大肠杆菌不耐热肠毒素反向被动胶凝集试验更灵敏，可检测粪便和水中的霍乱毒素。
7. Isolation and Characterization of a Vibrio cholerae Strain Unable to Secrete Cholera Toxin, and Cloning and Characterization of Genes from Vibrio cholerae that Confer a Congo Red Dye Binding Phenotype on Escherichia coli. [R] . Coker, C. 1996

机译：无法分泌霍乱毒素的霍乱弧菌菌株的分离和鉴定，以及霍乱弧菌基因的克隆和表征，该基因在大肠杆菌中具有刚果红染料结合表型。

The Liposome PCR Assay Is More Sensitive than the Vibrio cholerae Enterotoxin and Escherichia coli Heat-Labile Enterotoxin Reversed Passive Latex Agglutination Test at Detecting Cholera Toxin in Feces and Water

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