• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans
【2h】

Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans

机译：多重PCR鉴别感染人类的​​Broad虫（Cestoda：Diphyllobothrium）

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing, although highly precise, are too complicated, long, and expensive to be employed in medical laboratories for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA.
机译：通过形态学观察很难对感染人类的​​宽tape虫（Diphyllobothrium属）进行具体鉴定。通过PCR和测序进行的分子分析是迄今为止鉴定物种水平上这些寄生虫的唯一可靠工具。由于人类双叶硫菌病最近在几个国家传播，正确的诊断对于更好地了解人类感染物种的分布和生命周期以及防止寄生虫进入无病水系统至关重要。然而，PCR和测序尽管高度精确，却过于复杂，冗长且昂贵，以至于不能在医学实验室中用于常规诊断。在本研究中，我们基于多重PCR技术，通过一种廉价且快速的分子检测技术，用于鉴别感染人类的​​最常见双毛phyl属物种（D. latum，D。dendriticum，D。nihonkaiense和D. pacificum）。线粒体DNA的细胞色素c氧化酶亚基1基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号