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首页> 美国卫生研究院文献>Journal of Animal Science >The effect of orexin B on steroidogenic acute regulatory protein P450 side-chain cleavage enzyme and 3β-hydroxysteroid dehydrogenase gene expression and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the estrous cycle
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The effect of orexin B on steroidogenic acute regulatory protein P450 side-chain cleavage enzyme and 3β-hydroxysteroid dehydrogenase gene expression and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the estrous cycle

机译：食欲素B对早期妊娠和动情周期中猪子宫甾体生成的急性调节蛋白P450侧链裂解酶和3β-羟基甾体脱氢酶基因表达以及黄体酮和雄烯二酮分泌的影响

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The aim of this study was to investigate the effect of orexin B ( ) on progesterone (P ) and androstenedione (A ) secretion by porcine endometrial and myometrial tissue explants and on the expression of key steroidogenic proteins and enzymes involved in steroid production. The hormones secretion and the expression of steroidogenic acute regulatory protein ( ), P450 side-chain cleavage enzyme ( ), and 3β-hydroxysteroid dehydrogenase ( ) were analyzed on days 10 to 11, 12 to 13, 15 to 16, and 27 to 28 of pregnancy and during the luteal phase of the estrous cycle (days 10 to 11). Endometrial and myometrial explants were cultured in vitro in the presence of OXB (1, 10, or 100 nM) and OXB (1, 10, or 100 nM) with 1 µM of JNJ (OX2R antagonist). Gene expression was examined by real-time PCR, and steroid secretion was determined by radioimmunoassay. Orexin B modulated , , mRNA content depending on the type of uterine tissue, the applied OXB dose, and the stage of pregnancy or the estrous cycle ( < 0.05). Orexin B increased P secretion in all stages of early gestation ( < 0.05). Orexin B enhanced the release of A on days 12 to 13, 15 to 16, and 27 to 28 of gestation, whereas on days 10 to 11 of early pregnancy, A secretion decreased in the endometrium and increased in the myometrium ( < 0.05). These results indicate that OXB affects the expression of key steroidogenic regulators and the secretion of steroid hormones in the porcine uterus during early pregnancy.
机译：这项研究的目的是研究orexin B（）对猪子宫内膜和子宫肌层组织外植体分泌的孕酮（P）和雄烯二酮（A）的影响，以及与类固醇产生有关的关键类固醇生成蛋白和酶的表达。在第10至11、12至13、15至16和27至28天分析激素的分泌和类固醇生成的急性调节蛋白（），P450侧链裂解酶（）和3β-羟基类固醇脱氢酶（）的表达。怀孕和发情周期的黄体期（第10至11天）。子宫内膜和肌层外植体在OXB（1、10或100 nM）和OXB（1、10或100 nM）和1 µM JNJ（OX2R拮抗剂）存在下进行体外培养。通过实时PCR检查基因表达，并通过放射免疫测定法测定类固醇分泌。 Orexin B根据子宫组织的类型，所应用的OXB剂量以及妊娠阶段或发情周期来调节，mRNA含量（<0.05）。食欲素B在早期妊娠的所有阶段均增加P分泌（<0.05）。食欲素B在妊娠的第12至13、15至16和27至28天增强A的释放，而在早孕的第10至11天，子宫内膜的分泌减少而子宫肌层的分泌增加（<0.05）。这些结果表明OXB影响早期妊娠期间猪子宫中关键类固醇生成调节剂的表达和类固醇激素的分泌。

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