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首页> 美国卫生研究院文献>Journal of Animal Science >231 Urinary metabotyping around parturition indicates consistent metabolite signatures that can be used for monitoring and diagnosing of subclinical mastitis in dairy cows.
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231 Urinary metabotyping around parturition indicates consistent metabolite signatures that can be used for monitoring and diagnosing of subclinical mastitis in dairy cows.

机译：231分娩前后的尿代谢异常表明代谢物特征一致可用于监测和诊断奶牛的亚临床乳腺炎。

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The objective of this study was to identify urinary metabolite signatures that can be used for monitoring and diagnosing of subclinical mastitis in Holstein dairy cows. Six multiparous pregnant Holstein cows with subclinical mastitis (SCM) and 20 healthy controls (CON) were selected out of 100 cows in the study. DI/LC-MS/MS-based urinary analyses were conducted on samples collected at -8, -4, SCM diagnosis, and at +4 and +8 wks around the expected day of calving. Data indicated that 14 amino acids and derivatives were found in the urine with Gln and SDMA consistently increased ( <0.005) in the urine of SCM cows. Out of 39 acylcarnitines (ACs) quantified C12:DC, C14, and C18:2, were higher (P<0.05) in SCM at all time points (P<0.05). Seventeen ACs at -8 wks, 10 at -4 wks, 23 at disease wk, 8 at +4 wks, and 12 at +8 wks were higher (P<0.05) in the urine of SCM cows. Most of the alterations regarding lysophosphatidylcholines (LPCs) were found at -8 and -4 wks prepartum with 6 and 4 LPCs species lowered at -8 and -4 wks, respectively (P<0.05). Multiple sphingomyelin (SM) species prior to calving (3 at -8 wks and 8 at -4 wks) and 2 at disease week were lowered in SCM cows (P<0.05). A total of 74 phosphatidylcholines (PCs) were measured in the urine. A total of 12, 12, 10, 9, and 9 species of PC aa and 7, 15, 15, 3, and 3 species of PC ae were altered during weeks -8, -4, Disease wk, +4, + 8 wks around calving. Overall, multiple metabolite species related to amino acids, ACs, LPCs, and PCs metabolism were identified as altered in the urine of periparturient dairy cows. Some of the most important metabolites might be used as biomarkers to screen cows for SCM.
机译：这项研究的目的是确定可用于监测和诊断荷斯坦奶牛亚临床乳腺炎的尿代谢物特征。在这项研究中，从100头母牛中选择了6头患有亚临床乳腺炎（SCM）的多胎妊娠荷斯坦奶牛和20名健康对照（CON）。基于DI / LC-MS / MS的尿液分析是在预期产犊日的-8，-4，SCM诊断以及+4和+8 wks收集的样本上进行的。数据表明，在SCM奶牛的尿液中发现了14种氨基酸及其衍生物，其中Gln和SDMA持续增加（<0.005）。在所有时间点，在SCM中量化的39种酰基肉碱（AC）中C12：DC，C14和C18：2均较高（P <0.05）。在SCM母牛的尿液中，每周-8周的17个AC，在-4周的10个AC，在疾病周的23个AC，在+4周的8个AC和在+8周的12个AC更高（P <0.05）。关于溶血磷脂酰胆碱（LPCs）的大多数改变在产前-8和-4 wks被发现，其中6和4个LPCs分别在-8和-4 wks降低（P <0.05）。在SCM母牛中，产犊前的多种鞘磷脂（SM）种类（在-8周时为3个，在-4周时为8个）和在疾病周减少了2个（P <0.05）。尿液中共检测出74种磷脂酰胆碱（PC）。在-8，-4，疾病周，+ 4，+ 8周中，总共改变了12种，12种，10种，9种和9种PC aa和7种，15种，15种，3种和3种PC ae。周产犊。总体而言，鉴定出围产期奶牛尿液中与氨基酸，AC，LPC和PC代谢相关的多种代谢物种类。一些最重要的代谢物可以用作生物标志物，以筛选母牛的SCM。

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