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首页> 美国卫生研究院文献>Journal of Animal Science >128 Evaluation of SexedULTRA sex-sorted semen in timed artificial insemination programs for mature beef cows.
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128 Evaluation of SexedULTRA sex-sorted semen in timed artificial insemination programs for mature beef cows.

机译：128在定时的人工授精程序中对成熟肉牛进行SexedULTRA按性别分类的精液评估。

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An experiment was designed to compare SexedULTRA sex-sorted semen and conventional semen in either fixed-time (FTAI) or split-time artificial insemination (STAI) of suckled beef cows. Units of sex-sorted (4.0x10 cells/unit) and conventional (25.0x10 cells/unit) semen were produced using contemporaneous ejaculates from three bulls. Cows (n=1620) across four herds were treated with the 7-d CO-Synch+CIDR protocol [administration of gonadotropin-releasing hormone (GnRH) and insertion of a progesterone insert (CIDR) on Day -10, followed by administration of prostaglandin F (PG) and CIDR removal on Day -3]. Cows were preassigned based on age, body condition score, and days postpartum to receive either FTAI or STAI with either conventional or sex-sorted semen. On Day -3, estrus detection aids (Estrotect ) were applied. In FTAI treatments, AI was performed on Day 0 at 66h after PG administration and CIDR removal, and GnRH was administered concurrent with AI. In STAI treatments, AI was performed on Day 0 (66h) or Day 1 (90h) based on Estrotect activation, with 100 μg GnRH administered to cows failing to express estrus by 90h. Pregnancy rates were affected (P=0.04) by the interaction of bull x semen type. Greater pregnancy rates were obtained with conventional versus sex-sorted semen for Bull A (64% versus 36%; P =0.001) and Bull B (72% versus 57%; P<0.01), whereas pregnancy rates did not differ between conventional and sex-sorted semen for Bull C (58% versus 52%). Pregnancy rates did not differ between STAI and FTAI approaches. However, total estrous response was greater (P<0.001) using STAI (84%) compared to FTAI (72%), and estrous cows obtained greater pregnancy rates (P<0.0001). In summary, relative fertility of sex-sorted and conventional semen varied among bulls. Although pregnancy rates were similar in STAI and FTAI, the increased estrous response observed in STAI may be advantageous if restricting use of sex-sorted semen to estrous cows.
机译：设计了一个实验，比较固定奶牛的固定时间（FTAI）或分时人工授精（STAI）中的SexedULTRA按性别分类的精液和常规精液。使用来自三头公牛的同期射精产生了按性别分类（4.0x10个细胞/单位）和常规（25.0x10个细胞/单位）精液的单位。用7天CO-Synch + CIDR方案[促性腺激素释放激素（GnRH）的给药和第-10天的孕酮插入物（CIDR）的插入）对四头牛的母牛（n = 1620）进行处理。前列腺素F（PG）和CIDR在第-3天的清除]。根据年龄，身体状况评分和产后天数对母牛进行预分配，以接受FTAI或STAI常规或按性别分选的精液。在第-3天，使用发情检测辅助剂（Estrotect）。在FTAI治疗中，在PG给药和CIDR去除后第0天的第0天进行AI，并且GnRH与AI同时给药。在STAI处理中，基于Estrotect激活，在第0天（66h）或第1天（90h）进行了AI，对在90h内未发情的母牛给予100μgGnRH。公牛x精液类型的相互作用影响了妊娠率（P = 0.04）。公牛A（64％vs 36％； P = 0.001）和公牛B（72％vs 57％； P <0.01）的常规精液和性别精液的妊娠率更高，而常规和精液之间的妊娠率无差异按性别分类的公牛精液（58％对52％）。 STAI和FTAI方法之间的妊娠率没有差异。然而，与FTAI（72％）相比，使用STAI（84％）的总发情反应更大（P <0.001），发情的母牛获得更高的妊娠率（P <0.0001）。总之，公牛分性别和常规精液的相对生育能力各不相同。尽管STAI和FTAI中的妊娠率相似，但如果将按性别分类的精液限制用于发情的母牛，则STAI中观察到的发情反应增加可能是有利的。

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