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首页> 美国卫生研究院文献>Journal of Animal Science >59 Effect of heat processing on nutritive value of oat (Avena sativa L.) grain for dairy cattle as determined by DVE/OEB system and its relationship to oat grain protein molecular structure using advanced molecular spectroscopy.
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59 Effect of heat processing on nutritive value of oat (Avena sativa L.) grain for dairy cattle as determined by DVE/OEB system and its relationship to oat grain protein molecular structure using advanced molecular spectroscopy.

机译：59热处理对通过DVE / OEB系统测定的奶牛燕麦（Avena sativa L.）谷物营养价值的影响及其使用先进分子光谱技术与燕麦谷物蛋白质分子结构的关系。

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This study aimed to: (1) evaluate the heat processing effect on predicted nutrient supply to the small intestine (SI) in dairy cows; (2) detect associations between nutrient supply and protein molecular structure profiles of oat grain using Attenuated Total Reflectance Fourier transform molecular spectroscopy (ATR-FTIR). Oat grain was sampled from harvested plots (n=2) grown in 2014 and 2015. Each sample (1kg) was subsampled (4 portions) and each portion was subjected to one treatment: raw, dry heating (DH; air-draft oven 60min; 120°C); moist-heating (MH; autoclave at 1.05 kg/cmin situ trial to determine ruminal degradation kinetics. The DVE/OEB system was used to determine the degradability and digestibility of the nutrients, and rumen microbial protein synthesis. Results from the DVE/OEB system were correlated to protein molecular structure obtained using ATR-FTIR. Organic matter fermented in the rumen (FOM) and digested organic matter (DOM) were greater for DH and MIR (P0.05). High correlations were obtained between DVE and amide total area, amide I and II areas (AI; AII), amide I height (AIH), α-helix, β-sheet and α-helix:β-sheet, and between OEB and amide I:amide II ratio. In conclusion, heat processing improved the availability of OM in the rumen and CP in the SI from oat grain for dairy cows. The ATR-FTIR results could be used to predict nutrient supply in dairy cows.
机译：这项研究旨在：（1）评估热处理对奶牛小肠（SI）预测营养供应的影响； （2）使用衰减全反射傅里叶变换分子光谱（ATR-FTIR）检测燕麦谷物的营养供应与蛋白质分子结构特征之间的关联。从2014年和2015年种植的收获地（n = 2）中取样燕麦籽。对每个样本（1千克）进行二次取样（4份），然后对每份进行一次处理：生，干加热（DH；风炉60分钟） ; 120°C）;湿热（MH； 1.05 kg / cmin高压灭菌器，用于测定瘤胃降解动力学）。DVE/ OEB系统用于确定营养物质的降解性和消化率以及瘤胃微生物蛋白质的合成。DVE/ OEB系统的结果与ATR-FTIR得到的蛋白质分子结构相关，DH和MIR在瘤胃中发酵的有机物（FOM）和消化的有机物（DOM）更大（P0.05），DVE与酰胺总面积之间具有高度相关性。 ，酰胺I和II面积（AI; AII），酰胺I高度（AIH），α-螺旋，β-折叠和α-螺旋：β-折叠，以及OEB与酰胺I：酰胺II的比例之间。加工提高了奶牛燕麦中瘤胃中OM的利用率和SI中CP的利用率，ATR-FTIR结果可用于预测奶牛的营养供应。

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