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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray
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Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

机译：牛C组链球菌dysgalactiae亚种中检测到的毒力基因库。使用化脓性链球菌毒力微阵列分离出乳汁异乳症

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A custom-designed microarray containing 220 virulence genes of Streptococcus pyogenes (group A Streptococcus [GAS]) was used to test group C Streptococcus dysgalactiae subsp. dysgalactiae (GCS) field strains causing bovine mastitis and group C or group G Streptococcus dysgalactiae subsp. equisimilis (GCS/GGS) isolates from human infections, with the latter being used for comparative purposes, for the presence of virulence genes. All bovine and all human isolates carried a fraction of the 220 genes (23% and 39%, respectively). The virulence genes encoding streptolysin S, glyceraldehyde-3-phosphate dehydrogenase, the plasminogen-binding M-like protein PAM, and the collagen-like protein SclB were detected in the majority of both bovine and human isolates (94 to 100%). Virulence factors, usually carried by human beta-hemolytic streptococcal pathogens, such as streptokinase, laminin-binding protein, and the C5a peptidase precursor, were detected in all human isolates but not in bovine isolates. Additionally, GAS bacteriophage-associated virulence genes encoding superantigens, DNase, and/or streptodornase were detected in bovine isolates (72%) but not in the human isolates. Determinants located in non-bacteriophage-related mobile elements, such as the gene encoding R28, were detected in all bovine and human isolates. Several virulence genes, including genes of bacteriophage origin, were shown to be expressed by reverse transcriptase PCR (RT-PCR). Phylogenetic analysis of superantigen gene sequences revealed a high level (>98%) of identity among genes of bovine GCS, of the horse pathogen Streptococcus equi subsp. equi, and of the human pathogen GAS. Our findings indicate that alpha-hemolytic bovine GCS, an important mastitis pathogen and considered to be a nonhuman pathogen, carries important virulence factors responsible for virulence and pathogenesis in humans.
机译：定制设计的微阵列包含220个化脓性链球菌的毒力基因（A组链球菌[GAS]），用于测试C组链球菌性乳球菌亚种。引起牛乳腺炎和C组或G组链球菌痢疾半乳腺亚种的乳腺发育不良（GCS）野毒株。从人类感染中分离出的equimisimilis（GCS / GGS）分离株，后者用于存在毒性基因的比较目的。所有牛和人类分离株均携带220个基因的一部分（分别为23％和39％）。在大多数牛和人分离物中（94％至100％）都检测到了编码链球菌溶血素S，甘油醛-3-磷酸脱氢酶，纤溶酶原结合M样蛋白PAM和胶原样蛋白SclB的毒力基因。在所有人类分离株中均未检测到通常由人类β-溶血性链球菌病原体（如链激酶，层粘连蛋白结合蛋白和C5a肽酶前体）携带的毒力因子。此外，在牛分离株（72％）中检出了编码超抗原，DNase和/或链霉菌糖酶的GAS噬菌体相关毒力基因，但在人类分离株中未检出。在所有牛和人分离株中都检测到位于非噬菌体相关移动元件中的决定簇，例如编码R28的基因。逆转录酶PCR（RT-PCR）可以表达几种毒力基因，包括噬菌体起源的基因。对超抗原基因序列的系统进化分析表明，马病原体马链球菌亚种牛GCS基因之间的同一性高（> 98％）。以及人类病原体GAS。我们的发现表明，α-溶血性牛GCS是一种重要的乳腺炎病原体，被认为是非人类病原体，携带重要的毒力因子，负责人类的毒力和发病机理。

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