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首页> 美国卫生研究院文献>Journal of Cellular and Molecular Medicine >Intravenous administration of iPS‐MSCSPIONs mobilized into CKD parenchyma and effectively preserved residual renal function in CKD rat
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Intravenous administration of iPS‐MSCSPIONs mobilized into CKD parenchyma and effectively preserved residual renal function in CKD rat

机译：静脉内施用iPS‐MSCSPIONs进入CKD实质并有效保留CKD大鼠的残余肾功能

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This study traced intravenously administered induced pluripotent stem cell (iPSC)‐derived mesenchymal stem cells (MSC) and assessed the impact of iPSC‐MSC on preserving renal function in SD rat after 5/6 nephrectomy. The results of in vitro study showed that FeraTrack™Direct contrast particles (ie intracellular magnetic labelling) in the iPSC‐MSC (ie iPS‐MSC ) were clearly identified by Prussian blue stain. Adult‐male SD rats (n = 40) were categorized into group 1 (SC), group 2 [SC + iPS‐MSC (1.0 × 10 cells)/intravenous administration post‐day‐14 CKD procedure], group 3 (CKD), group 4 [CKD + iPS‐MSC (0.5 × 10 cells)] and group 5 [CKD + iPS‐MSC (1.0 × 10 cells)]. By day‐15 after CKD induction, abdominal MRI demonstrated that iPS‐MSC were only in the CKD parenchyma of groups 4 and 5. By day 60, the creatinine level/ratio of urine protein to urine creatinine/kidney injury score (by haematoxylin and eosin stain)/fibrotic area (Masson's trichrome stain)/IF microscopic finding of kidney injury molecule‐1 expression was lowest in groups 1 and 2, highest in group 3, and significantly higher in group 4 than in group 5, whereas IF microscopic findings of podocyte components (ZO‐1/synaptopodin) and protein levels of anti‐apoptosis ((Bad/Bcl‐xL/Bcl‐2) exhibited an opposite pattern to creatinine level among the five groups (all  P SPIONs that were identified only in CKD parenchyma effectively protected the kidney against CKD injury.
机译：这项研究追踪了静脉内施用的诱导多能干细胞（iPSC）衍生的间充质干细胞（MSC），并评估了iPSC-MSC对5/6肾切除术后SD大鼠保存肾脏功能的影响。体外研究结果表明，普鲁士蓝染色可清楚地识别iPSC-MSC（即iPS-MSC）中的FeraTrack™Direct对比颗粒（即细胞内磁性标记）。成年雄性SD大鼠（n = 40）分为第1组（SC），第2组[SC + iPS-MSC（1.0×10个细胞）/第14天CKD术后静脉给药]，第3组（CKD） ，第4组[CKD + iPS‐MSC（0.5×10个细胞）]和第5组[CKD + iPS‐MSC（1.0×10个细胞）]。 CKD诱导后第15天，腹部MRI证实iPS‐MSC仅在第4组和第5组的CKD实质中。到第60天，肌酐水平/尿蛋白与尿肌酐/肾损伤分数的比率（由苏木精和曙红染色）/纤维化区域（马森三色染色）/ IF显微镜下发现肾损伤分子-1在第1组和第2组中最低，在第3组中最高，在第4组中显着高于第5组，而IF显微镜下发现五组中足细胞成分（ZO-1 /突触足蛋白）和抗凋亡蛋白水平（（Bad / Bcl-xL / Bcl-2）的表达与肌酐水平相反（仅在CKD中发现的所有P SPIONs）实质保护肾脏免受CKD损伤。

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