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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >LysGH15 a Novel Bacteriophage Lysin Protects a Murine Bacteremia Model Efficiently against Lethal Methicillin-Resistant Staphylococcus aureus Infection
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LysGH15 a Novel Bacteriophage Lysin Protects a Murine Bacteremia Model Efficiently against Lethal Methicillin-Resistant Staphylococcus aureus Infection

机译：LysGH15一种新型噬菌体溶素可有效保护鼠类细菌血症模型抵抗致死性耐甲氧西林的金黄色葡萄球菌感染

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摘要

Phage-coded lysin is an enzyme that destroys the cell walls of bacteria. Phage lysin could be an alternative to conventional antibiotic therapy against pathogens that are resistant to multiple antibiotics. In this study, a novel staphylococcal phage, GH15, was isolated, and the endogenous lytic enzyme (LysGH15) was expressed and purified. The lysin LysGH15 displayed a broad lytic spectrum; in vitro treatment killed a number of Staphylococcus aureus strains rapidly and completely, including methicillin-resistant S. aureus (MRSA). In animal experiments, a single intraperitoneal injection of LysGH15 (50 μg) administered 1 h after MRSA injections at double the minimum lethal dose was sufficient to protect mice (P < 0.01). Bacteremia in unprotected mice reached colony counts of about 107 CFU/ml within 3.5 h after challenge, whereas the mean colony count in lysin-protected mice was less than 104 CFU/ml (and ultimately became undetectable). These results indicate that LysGH15 can kill S. aureus in vitro and can protect mice efficiently from bacteremia in vivo. The phage lysin LysGH15 might be an alternative treatment strategy for infections caused by MRSA.
机译：噬菌体编码的溶素是一种破坏细菌细胞壁的酶。噬菌体溶素可以替代传统的抗多种病原体的抗生素疗法。在这项研究中，分离出一种新型的葡萄球菌噬菌体GH15，并表达和纯化了内源性裂解酶（LysGH15）。溶菌素LysGH15表现出宽广的裂解光谱。体外治疗迅速，完全杀死了许多金黄色葡萄球菌菌株，包括耐甲氧西林的金黄色葡萄球菌（MRSA）。在动物实验中，MRSA注射后1小时以最小致死剂量的两倍腹膜内注射LysGH15（50μg）足以保护小鼠（P <0.01）。攻击后3.5小时内，未保护小鼠的菌血症菌落数达到约10 7 CFU / ml，而溶菌素保护小鼠的菌落数均值低于10 4 CFU / ml（最终无法检测到）。这些结果表明LysGH15可以在体外杀死金黄色葡萄球菌，并且可以在体内有效地保护小鼠免于细菌血症。噬菌体溶素LysGH15可能是由MRSA引起的感染的另一种治疗策略。

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