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首页> 美国卫生研究院文献>Membranes >How Molecular Weight Cut-Offs and Physicochemical Properties of Polyether Sulfone Membranes Affect Peptide Migration and Selectivity during Electrodialysis with Filtration Membranes
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How Molecular Weight Cut-Offs and Physicochemical Properties of Polyether Sulfone Membranes Affect Peptide Migration and Selectivity during Electrodialysis with Filtration Membranes

机译：聚醚砜膜的截留分子量和理化性质如何影响电渗滤膜的肽迁移和选择性。

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Filtration membranes (FMs) are an integral part of electrodialysis with filtration membranes (EDFM), a green and promising technology for bioactive peptide fractionation. Therefore, it is paramount to understand how physicochemical properties of FMs impact global and selective peptide migration to anionic (A ) and cationic (C ) peptide recovery compartments during their simultaneous separation by EDFM. In this context, six polyether sulfone (PES) membranes with molecular weight cut-offs (MWCO) of 5, 10, 20, 50, 100 and 300 kDa were characterized and used during EDFM to separate peptides from a complex whey protein hydrolysate. Surface charge, roughness, thickness and surface/pores nature of studied PES membranes were similar with small differences in conductivity, porosity and pore size distribution. Interestingly, global peptides migration to both recovery compartments increased linearly as a function of MWCO. However, peptide selectivity changed according to the recovery compartments and/or the peptide’s charge and MW with an increase in MWCO of FMs. Indeed, in A , the relative abundance (RA) of peptides having low negative charge and MW (IDALNENK and VLVLDTDYK) decreased (45% to 19%) with an increase in MWCO, while the opposite for peptides having high negative charge and MW (TPEVDDEALEK, TPEVDDEALEKFDK & VYVEELKPTPEGDLEILLQK) (increased from 16% to 43%). Concurrently, in C , regardless of MWCO used, the highest RA was observed for peptides having low positive charge and MW (IPAVFK & ALPMHIR). It was the first time that the significant impact of charge, MWCO and pore size distribution of PES membranes on a wide range of MWCO was demonstrated on EDFM performances.
机译：过滤膜（FMs）与过滤膜（EDFM）是电渗析不可或缺的一部分，这是一种绿色且有前途的生物活性肽分级分离技术。因此，至关重要的是要了解FM的理化特性如何影响在通过EDFM同时分离过程中，整体和选择性肽向阴离子（A）和阳离子（C）肽回收区的迁移。在本文中，表征了六种分子量截断值（MWCO）为5、10、20、50、100和300 kDa的聚醚砜（PES）膜，并在EDFM中用于从复杂的乳清蛋白水解物中分离出肽。研究的PES膜的表面电荷，粗糙度，厚度和表面/孔性质相似，但电导率，孔隙率和孔径分布差异很小。有趣的是，作为MWCO的函数，整体肽向两个回收区的迁移呈线性增加。但是，随着FM的MWCO的增加，肽的选择性会根据回收区和/或肽的电荷和分子量而变化。实际上，在A中，具有较低负电荷和MW的肽（IDALNENK和VLVLDTDYK）的相对丰度（RA）随着MWCO的增加而降低（45％至19％），而具有高负电荷和MW的肽的相对丰度（RA）却相反（ TPEVDDEALEK，TPEVDDEALEKFDK和VYVEELKPTPEGDLEILLQK）（从16％增加到43％）。同时，在C中，无论使用何种MWCO，对于具有低正电荷和MW的肽（IPAVFK和ALPMHIR），观察到最高的RA。 EDFM性能首次证明了电荷，MWCO和PES膜的孔径分布对多种MWCO的显着影响。

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