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Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis

机译:猪粪与玉米秸秆的长期中温厌氧共消化和微生物群落分析

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摘要

Long-term anaerobic co-digestion of swine manure (SM) and corn stover (CS) was conducted using semi-continuously loaded digesters under mesophilic conditions. A preliminary test was first conducted to test the effects of loading rates, and results indicated the 3 g-VS L d was the optimal loading rate. Based on the preliminary results, a verification replicated test was conducted with 3 g-VS L d loading rate and different SM/CS ratios (1:1, 2:1 and 1:2). Results showed that a SM/CS ratio of 2/1 was optimal, based on maximum observed methane-VS generation and carbon conversion efficiency (72.56 ± 3.40 mL g and 40.59%, respectively). Amplicon sequencing analysis suggested that microbial diversity was increased with CS loading. Amino-acid-degrading bacteria were abundant in the treatment groups. Archaea could enhance biogas and methane productions.
机译:猪粪(SM)和玉米秸秆(CS)的长期厌氧消化是在中温条件下使用半连续消化池进行的。首先进行初步测试以测试加载速率的影响,结果表明3 g-VS L d是最佳加载速率。根据初步结果,以3 g-VS L d的加载速率和不同的SM / CS比(1:1、2:1和1:2)进行了重复验证试验。结果表明,基于观察到的最大甲烷-VS生成和碳转化效率(分别为72.56±3.40 mL g和40.59%),SM / CS比为2/1是最佳的。扩增子测序分析表明,随着CS负载,微生物多样性增加。治疗组中氨基酸降解细菌丰富。古细菌可以提高沼气和甲烷的产量。

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