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首页> 美国卫生研究院文献>Microorganisms >Insights into Biodegradation Related Metabolism in an Abnormally Low Dissolved Inorganic Carbon (DIC) Petroleum-Contaminated Aquifer by Metagenomics Analysis
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Insights into Biodegradation Related Metabolism in an Abnormally Low Dissolved Inorganic Carbon (DIC) Petroleum-Contaminated Aquifer by Metagenomics Analysis

机译：代谢组学分析异常低溶解的无机碳（DIC）石油污染的含水层中的生物降解相关的代谢组学分析。

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In petroleum-contaminated aquifers, biodegradation is always associated with various types of microbial metabolism. It can be classified as autotrophic (such as methanogenic and other carbon fixation) and heterotrophic (such as nitrate/sulfate reduction and hydrocarbon consumption) metabolism. For each metabolic type, there are several key genes encoding the reaction enzymes, which can be identified by metagenomics analysis. Based on this principle, in an abnormally low dissolved inorganic carbon (DIC) petroleum-contaminated aquifer in North China, nine groundwater samples were collected along the groundwater flow, and metagenomics analysis was used to discover biodegradation related metabolism by key genes. The major new finding is that autotrophic metabolism was revealed, and, more usefully, we attempt to explain the reasons for abnormally low DIC. The results show that the methanogenesis gene, , was undetected but more carbon fixation genes than nitrate reduction and sulfate genes were found. This suggests that there may be a considerable number of autotrophic microorganisms that cause the phenomenon of low concentration of dissolved inorganic carbon in contaminated areas. The metagenomics data also revealed that most heterotrophic, sulfate, and nitrate reduction genes in the aquifer were assimilatory sulfate and dissimilatory nitrate reduction genes. Although there was limited dissolved oxygen, aerobic degrading genes and were more abundant than anaerobic degrading genes and . The metagenomics information can enrich our microorganic knowledge about petroleum-contaminated aquifers and provide basic data for further bioremediation.
机译：在受石油污染的含水层中，生物降解总是与各种类型的微生物代谢有关。它可以分为自养（如甲烷生成和其他碳固定）和异养（如硝酸盐/硫酸盐还原和碳氢化合物消耗）代谢。对于每种代谢类型，都有几个编码反应酶的关键基因，可以通过宏基因组学分析来鉴定。基于这一原理，在华北异常低溶解的无机碳（DIC）石油污染的含水层中，沿地下水流收集了九个地下水样品，并进行了宏基因组分析，发现了关键基因与生物降解相关的代谢。主要的新发现是揭示了自养代谢，更有用的是，我们试图解释DIC异常低的原因。结果表明，未发现产甲烷基因，但发现的碳固定基因比硝酸盐还原和硫酸盐基因更多。这表明可能存在大量自养微生物，导致受污染地区的溶解性无机碳浓度低。宏基因组学数据还显示，含水层中大多数异养，硫酸盐和硝酸盐还原基因是同化硫酸盐和异化硝酸盐还原基因。尽管溶解氧有限，但好氧降解基因和比厌氧降解基因和丰富。宏基因组学信息可以丰富我们对石油污染含水层的微生物知识，并为进一步的生物修复提供基础数据。

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