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Fodinicola acaciae sp. nov. an Endophytic Actinomycete Isolated from the Roots of Acacia mangium Willd. and Its Genome Analysis

机译:福金尼科植物十一月从马占相思的根中分离出来的内生放线菌。及其基因组分析

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摘要

A novel endophytic actinomycete strain GKU 173 isolated from the roots of Willd. showed potential plant growth promoting (PGP) activity. Phylogenetic analysis, based on 16S rRNA gene, indicated that strain GKU 173 was the most closely related to HKI 0501 —the only species in the genus . Morphology and chemotaxonomy of strain GKU 173 indicated that it belongs to the genus by having meso-diaminopimelic acid in the cell wall and xylose as the characteristic cell-wall sugars. The cellular fatty acid profile mainly comprised iso-C , anteiso-C , iso-C , and iso-C . The major menaquinones were MK-9(H ), MK-9(H ), and MK-9(H ). The main polar phospholipids contained diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylinositol (PI). Genome analysis signified DNA G+C content of 67.81 mol%. The level of digital DNA-DNA relatedness between strain GKU 173 and the type strain was 21.30%. On the basis of polyphasic characteristics, strain GKU 173 clearly represents a novel species of the genus , for which the name sp. nov. (= TBRC 10620 = NBRC 114213 ) is proposed. Furthermore, genome analysis of both strains suggested that members of the genus are promising sources of beneficial PGP-actinomycetes and novel secondary metabolites.
机译:从Will的根中分离出的新型内生放线菌菌株GKU 173。显示出潜在的植物生长促进(PGP)活性。基于16S rRNA基因的系统发育分析表明,菌株GKU 173与HKI 0501密切相关-HKI 0501是该属中唯一的物种。 GKU 173菌株的形态和化学分类学表明,它属于细胞壁微生物,其中木糖为中二氨基己二酸,而木糖为特征细胞壁糖。细胞脂肪酸谱主要包括iso-C,anteiso-C,iso-C和iso-C。主要的甲萘醌为MK-9(H),MK-9(H)和MK-9(H)。主要的极性磷脂包含二磷脂酰甘油(DPG),磷脂酰乙醇胺(PE)和磷脂酰肌醇(PI)。基因组分析表明DNA G + C含量为67.81 mol%。 GKU 173菌株与典型菌株之间的数字DNA-DNA相关性水平为21.30%。基于多相特性,GKU 173菌株清楚地代表了该属的一个新物种,其名称为 sp。十一月(= TBRC 10620 = NBRC 114213)。此外,对这两个菌株的基因组分析表明,该属的成员是有益的PGP放线菌和新型次生代谢物的有前途的来源。

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