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Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

机译:平菇平菇漆酶基因在巴斯德毕赤酵母中的表达及其对玉米秸木质素的降解

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摘要

is a species of white-rot fungi that effectively degrades lignin. In this study, we aimed to efficiently express the gene of in the X33 yeast strain. The enzymatic properties of recombinant yeast were determined, and its ability to degrade corn stover lignin was determined. The results showed the optimum pH values of recombinant laccase for 2,2’-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid, 2,6-dimethoxyphenol, and 2-methoxyphenol were 3.0, 3.0, and 3.5, respectively. The optimum reaction temperature was 50 °C, and it had good thermal stability and acid and alkali resistance. The degradation rate of lignin in corn stover by recombinant laccase was 18.36%, and the native degradation rate was 14.05%, the difference between them is significant ( < 0.05). This experiment lays a foundation for the study of the degradation mechanism of lignin by laccase.
机译:是一种能够有效降解木质素的白腐真菌。在这项研究中,我们旨在在X33酵母菌株中有效表达的基因。确定了重组酵母的酶学性质,并确定了其降解玉米秸秆木质素的能力。结果表明,重组漆酶对2,2'-Azinobis-3-乙基苯并噻唑啉-6-磺酸,2,6-二甲氧基苯酚和2-甲氧基苯酚的最适pH值分别为3.0、3.0和3.5。最佳反应温度为50℃,具有良好的热稳定性和耐酸碱性能。重组漆酶在玉米秸秆中木质素的降解率为18.36%,天然降解率为14.05%,两者之间差异有统计学意义(<0.05)。该实验为研究漆酶降解木质素的机理奠定了基础。

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