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首页> 美国卫生研究院文献>Molecules >Preparation of Lignan-Rich Extract from the Aerial Parts of Phyllanthus niruri Using Nonconventional Methods
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Preparation of Lignan-Rich Extract from the Aerial Parts of Phyllanthus niruri Using Nonconventional Methods

机译：非常规方法从楠竹地上部分提取富木质素的提取物

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Phyllanthin and related lignans were found to be responsible, at least in part, for most of the activity of species. This observation encouraged the authors to develop methods for the preparation of an extract rich in phyllanthin and related lignans from the aerial parts of L. Direct extraction with solvents produced extracts with variable yields and contents of lignans. Lignans were identified by LC-ESI-MS analysis as phyllanthin (used as marker substance), hypophyllanthin, phylltetralin, nirtetralin, and niranthin. Extraction with boiling water produced 18.10 g% ( / ) extract with a trace amount of lignans (phyllanthin content of 0.33 ± 0.10 mg/g extract), while extraction with MeOH gave 3.6 g% / extract with a low phyllanthin content (3.1 mg/g extract), as determined by HPLC. However, Soxhlet extraction with hexane, CH Cl , or acetone gave extracts with low yields (0.82, 1.12, and 3.40 g% / , respectively) and a higher phyllanthin contents (36.2 ± 2.6, 11.7 ± 1.68, and 11.7 ± 1.10 mg/g extract, respectively). Extraction quality and efficiency were optimized by adopting the following three different approaches: (1) Alkaline digestion of the plant material with 30% potassium hydroxide yielded 3.1 g% / of purified extract with high phyllanthin content (22.34 ± 0.13 mg/g); (2) microwave-assisted extraction using 80% MeOH gave an extract with a better yield (8.13 g% / ) and phyllanthin content (21.2 ± 1.30 mg/g) (after filtration through a Diaion HP-20 column); and (3) treatment of the ground plant material at 50 °C with two hydrolytic enzymes, cellulase (9 U/g for 12 h) and then, protease (4 U/g up to 72 h) optimized the yield of extract (13.92 g% / ) and phyllanthin content (25.9 mg/g extract and total lignans content of 85.87 mg/g extract). In conclusion, the nonconventional methods presented here are superior for optimizing the yield of extract and its lignan contents from the aerial parts of .
机译：发现叶绿素和相关木脂素至少部分负责物种的大部分活动。这一观察结果鼓舞了作者开发出从L的地上部分制备富含phyllanthin和相关木脂素的提取物的方法。用溶剂直接提取可得到产量和木脂素含量可变的提取物。木质素通过LC-ESI-MS分析鉴定为叶绿素（用作标记物质），次叶绿素，叶绿素，尼尔富林和烟碱。用沸水萃取可得到18.10 g％（/）的萃取物和痕量的木脂素（葵花胶的含量为0.33±0.10 mg / g萃取物），而用MeOH萃取可得到3.6 g％的萃取物和低含量的树胶素（3.1 mg / g）。 g提取物），通过HPLC测定。但是，用己烷，CH Cl或丙酮进行索氏提取的提取物收率低（分别为0.82、1.12和3.40 g％/）和较高的花胶素含量（36.2±2.6、11.7±1.68和11.7±1.10 mg / g提取物）。通过以下三种不同的方法优化了提取质量和效率：（1）用30％的氢氧化钾对植物材料进行碱性消化，得到3.1 g％/提纯的高提取素（22.34±0.13 mg / g）； （2）使用80％MeOH的微波辅助萃取得到的萃取物收率更高（8.13 g％/），菊磷脂含量（21.2±1.30 mg / g）（通过Diaion HP-20柱过滤后）; （3）在50°C下用两种水解酶纤维素酶（9 U / g持续12 h）处理蛋白酶，然后用蛋白酶（4 U / g直至72 h）处理提取的植物材料（13.92）。 g％/）和phyllanthin含量（提取物25.9 mg / g，总木脂素含量为85.87 mg / g）。总之，本文提出的非常规方法对于从大豆地上部分提取物的提取物及其木脂素含量的优化具有优势。

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