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Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus

机译：Nuphar lutea提取物表现出对麻疹病毒的抗病毒活性

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Different parts of . (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.
机译：的不同部分。 （黄色睡莲）已用于治疗几种炎症和病原体相关疾病。研究表明，提取物（NUP）对多种病原体具有活性，包括细菌，真菌和利什曼原虫。为了检测针对负链RNA（-RNA）病毒的新型治疗剂，我们测试了Nuphar lutea叶的部分纯化生物碱混合物对麻疹病毒（MV）的影响。 MV疫苗的Edmonston菌株用于急性或持续感染细胞。通过蛋白质印迹和免疫细胞化学检测了几种MV蛋白的水平。通过qRT-PCR定量病毒RNA。通过感染非洲绿猴肾VERO细胞的单层细胞来监测病毒的感染性。我们表明NUP保护细胞免受急性感染。与未经处理的细胞相比，在持续感染的细胞中观察到MV P，N和V蛋白的减少，并且释放的感染性病毒数量减少了。通过检查病毒RNA，我们建议NUP在转录后水平起作用。我们得出结论，作为概念的证明，NUP具有针对MV的抗病毒治疗活性。未来的研究将确定作用机制以及NUP对其他相关病毒的作用。

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