首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Misidentification of a Rare Species Cryptococcus laurentii by Commonly Used Commercial Biochemical Methods and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems: Challenges for Clinical Mycology Laboratories
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Misidentification of a Rare Species Cryptococcus laurentii by Commonly Used Commercial Biochemical Methods and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems: Challenges for Clinical Mycology Laboratories

机译:常用商业生化方法和基质辅助激光解吸电离的飞行时间质谱系统对稀有物种劳伦隐球菌的误认:对临床真菌学实验室的挑战

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摘要

Forty-two putative Cryptococcus laurentii isolates identified by the Vitek 2 system were collected in China. The gold standard, internal transcribed spacer (ITS) sequencing, confirmed that only two isolates were genuine C. laurentii. Bruker Biotyper matrix-assisted laser desorption ionization–time of flight mass spectrometry was able to identify the C. laurentii isolates with an expanded custom database.
机译:在中国收集了通过Vitek 2系统鉴定的42种假定的劳伦隐球菌。金标准,内部转录间隔区(ITS)测序,证实只有两个分离株是真正的劳氏梭状芽胞杆菌。 Bruker Biotyper基质辅助激光解吸电离-飞行时间质谱仪能够通过扩展的自定义数据库来鉴定劳伦梭菌。

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