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In Vitro Propagation of Gastrochilus matsuran (Makino) Schltr. an Endangered Epiphytic Orchid

机译:濒危的附生兰花Gastrochilus matsuran(Makino)Schltr。的离体繁殖

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摘要

(Makino) Schltr. (Orchidaceae) populations are declining quickly because of overexploitation, climatic changes, and deforestation; therefore, mass-production protocols are required for this orchid. Natural propagation of this species is often hampered by meager seed germination and slow growth. Thus, our aim was to establish an effective protocol for the in vitro propagation of and reduce the risk of its extinction. We investigated the impacts of culture media, coconut water (CW), and plant hormones (gibberellic acid (GA ), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA), and thidiazuron (TDZ)) on asymbiotic germination, multiplication and conversion of protocorms, and plantlet development. Maximal seed germination (93.3%) was achieved on ½ MS medium without vitamins plus 5% CW, 1 µM NAA, and 1.5 µM GA . Secondary protocorm formation was best achieved on ½ MS medium without vitamins plus 2 µM TDZ. The conversion of protocorms into seedlings was maximized by supplementation with 2 µM IBA or 1 µM NAA. Acclimatized plantlets that exhibited exuberant growth on sphagnum moss were reintroduced to tree trunks in a natural habitat, with a 67% survival rate. This in vitro propagation procedure would be helpful for the mass production and conservation of this rare epiphytic orchid.
机译:(Makino)Schltr。 (兰科)人口由于过度开发,气候变化和森林砍伐而迅速减少。因此,此兰花需要量产协议。该物种的自然繁殖常常因种子发芽少和生长缓慢而受阻。因此,我们的目的是建立一个有效的方案,以在体外繁殖并降低其灭绝的风险。我们调查了培养基,椰子汁(CW)和植物激素(赤霉素(GA),吲哚-3-乙酸(IAA),吲哚-3-丁酸(IBA),α-萘乙酸(NAA)的影响)和噻唑隆(TDZ))在非共生萌发,原球茎的繁殖和转化以及小植株发育方面。在不含维生素加5%CW,1 µM NAA和1.5 µM GA的½MS培养基上可获得最大的种子发芽率(93.3%)。在没有维生素加2 µM TDZ的½MS培养基上,初生原球茎形成最好。通过添加2 µM IBA或1 µM NAA,可以最大限度地将原球茎转化为幼苗。在泥炭藓上生长旺盛的驯化小植株被重新引入自然栖息地的树干中,成活率为67%。这种体外繁殖程序将有助于这种稀有附生兰花的大量生产和保存。

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