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首页> 美国卫生研究院文献>Polymers >Evaluation of Different Methods for Cultivating Gluconacetobacter hansenii for Bacterial Cellulose and Montmorillonite Biocomposite Production: Wound-Dressing Applications
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Evaluation of Different Methods for Cultivating Gluconacetobacter hansenii for Bacterial Cellulose and Montmorillonite Biocomposite Production: Wound-Dressing Applications

机译：汉逊氏糖杆菌培养细菌纤维素和蒙脱土生物复合材料生产的不同方法的评价：伤口敷料应用

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Bacterial cellulose (BC) has received considerable attention due to its unique properties, including an ultrafine network structure with high purity, mechanical strength, inherent biodegradability, biocompatibility, high water-holding capacity and high crystallinity. These properties allow BC to be used in biomedical and industrial applications, such as medical product. This research investigated the production of BC by ATCC 23769 using different carbon sources (glucose, mannitol, sucrose and xylose) at two different concentrations (25 and 50 g∙L ). The BC produced was used to develop a biocomposite with montmorillonite (MMT), a clay mineral that possesses interesting characteristics for enhancing BC physical-chemical properties, at 0.5, 1, 2 and 3% concentrations. The resulting biocomposites were characterized in terms of their physical and barrier properties, morphologies, water-uptake capacities, and thermal stabilities. Our results show that bacteria presented higher BC yields in media with higher glucose concentrations (50 g∙L ) after a 14-day incubation period. Additionally, the incorporation of MMT significantly improved the mechanical and thermal properties of the BC membranes. The degradation temperature of the composites was extended, and a decrease in the water holding capacity (WHC) and an improvement in the water release rate (WRR) were noted. Determining a cost-effective medium for the production of BC and the characterization of the produced composites are extremely important for the biomedical applications of BC, such as in wound dressing materials.
机译：细菌纤维素（BC）由于其独特的性能而备受关注，包括具有高纯度，机械强度，固有的生物降解性，生物相容性，高持水能力和高结晶度的超细网络结构。这些特性使BC可以用于生物医学和工业应用，例如医疗产品。这项研究调查了ATCC 23769使用两种不同浓度（25和50 g∙L）的碳源（葡萄糖，甘露醇，蔗糖和木糖）生产BC的情况。产生的BC用于开发与蒙脱石（MMT）的生物复合材料，蒙脱土是一种粘土矿物，在0.5、1、2和3％的浓度下，具有增强BC物理化学性能的有趣特征。所得生物复合材料的物理和阻隔性能，形态，吸水能力和热稳定性均得到了表征。我们的结果表明，在14天的孵育期后，细菌在葡萄糖浓度较高（50 g∙L）的培养基中呈现较高的BC产量。另外，MMT的结合显着改善了BC膜的机械和热性能。复合材料的降解温度延长，并且持水量（WHC）降低和水释放速率（WRR）有所提高。确定用于生产BC的经济有效的介质并表征所生产的复合材料对于BC的生物医学应用（例如在伤口敷料中）极为重要。

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