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首页> 美国卫生研究院文献>Journal of Clinical Laboratory Analysis >Influence of pigments and pH of urine on the determination of N‐acetyl‐β‐d‐glucosaminidase activity with 2‐methoxy‐4‐(2′‐nitrovinyl)‐phenyl‐N‐acetyl‐β‐d‐glucosaminide
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Influence of pigments and pH of urine on the determination of N‐acetyl‐β‐d‐glucosaminidase activity with 2‐methoxy‐4‐(2′‐nitrovinyl)‐phenyl‐N‐acetyl‐β‐d‐glucosaminide

机译：尿液中色素和pH值对2-甲氧基-4-（2-硝基乙烯基）-苯基-N-乙酰基-β-d-氨基葡萄糖测定N-乙酰基-β-d-氨基葡萄糖苷酶活性的影响

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The influence of urinary pigments and urine pH on the spectrophotometric determination of ‐acetyl‐β‐ ‐glucosaminidase (NAG; EC 3.2.1.30) activity with 2‐methoxy‐4‐(2′‐nitrovinyl)‐phenyl‐ ‐acetyl‐β‐ ‐glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2‐methoxy‐4‐(2′‐nitrovinyl)‐phenol (MNP) at 505 nm. It was shown that besides the maximum (I) in the range of 350–360 nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380–460 nm. With the increase of pH, maximum II was shifted toward higher wavelengths and contributed to MNP absorption (5–90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495–400 nm) with increasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed. J. Clin. Lab. Anal. 19:260–266, 2005. © 2005 Wiley‐Liss, Inc.
机译：尿色素和尿液pH对分光光度法测定2-甲氧基-4-（2'-硝基乙烯基）-苯基-乙酰基-β-氨基葡萄糖苷酶（NAG; EC 3.2.1.30）活性的影响以氨基葡萄糖为底物进行了研究。研究是用人和兔子的尿液样本进行的。已发现碱性尿液pH值以两种方式影响NAG活性：1）NAG活性由于酶的不稳定性随pH的升高而降低，以及2）NAG活性的提高是由于尿色素对2-甲氧基-4- （2'-硝基乙烯基）-苯酚（MNP）在505nm处。结果表明，除了碱性尿液吸收光谱在350-360 nm范围内的最大值（I）之外，在380-460 rangenm范围内还有最大值（II）。随着pH值的增加，最大II移向更高的波长并有助于MNP吸收（5-90％）。另一方面，随着pH值的增加，MNP的吸收最大值移向了较低的波长（495-400nm）。提出了两种消除尿色素影响的方法。讨论了对人和兔子尿液中的NAG活性值进行校正的理由（研究镉的毒性作用的模型系统）。 J.临床实验室肛门19：260–266，2005.©2005 Wiley-Liss，Inc.

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