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首页> 美国卫生研究院文献>Journal of Clinical Laboratory Analysis >Development of a new method for measuring total urinary protein using acid violet 6B pigment
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Development of a new method for measuring total urinary protein using acid violet 6B pigment

机译：开发一种使用酸性紫6B颜料测定尿总蛋白的新方法

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We have developed a new method for measuring total urinary protein using acid violet 6B (AV6B) pigment. Nine purified components of human urinary proteins and urine samples collected randomly from 123 diabetic outpatients were used. There were 62, 36, and 25 cases of prenephropathy, early nephropathy, and overt nephropathy, respectively. All samples were measured by Coomassie brilliant blue G 250 (CBB), pyrogallol red‐molybdate (PRM), and AV6B methods using an optical photometer. In healthy subjects, the major components of urinary proteins, such as γ‐globulins, IgG, IgA Tamm‐Horsfall protein, and transferrin, the reactivity values of the AV6B and PRM methods were similar. The CBB method was the least sensitive of the three methods. In the urine samples from diabetic patients, the urinary protein values measured by the AV6B method were higher than those measured by the CBB method in the prenephropathy stage. The values obtained by the AV6B method (y) correlated well with those from the CBB method (x) (y = 1.243x+ 3.61, r = 0.904). When the values from the AV6B method (y) were compared to those from the PRM method (x), correlation was low (y = 1.406x−29.15, r = 0.786). In conclusion, the AV6B method was more useful than the CBB and PRM methods for low levels of urinary protein. J. Clin. Lab. Anal. 17: 147–154, 2003. © 2003 Wiley‐Liss, Inc.
机译：我们已经开发出一种使用酸性紫6B（AV6B）色素测量总尿蛋白的新方法。使用了从123位糖尿病门诊患者中随机采集的9种纯化的人尿蛋白成分和尿液样本。分别有62例，36例和25例前肾病，早期肾病和明显的肾病。所有样品均通过考马斯亮蓝G 250（CBB），邻苯三酚红钼酸盐（PRM）和AV6B方法使用光度计测量。在健康受试者中，尿蛋白的主要成分，例如γ-球蛋白，IgG，IgA Tamm-Horsfall蛋白和转铁蛋白，AV6B和PRM方法的反应性值相似。 CBB方法是这三种方法中最不敏感的。在糖尿病前期患者的糖尿病患者尿液中，通过AV6B方法测量的尿蛋白值高于通过CBB方法测量的尿蛋白值。通过AV6B方法（y）获得的值与从CBB方法（x）获得的值具有很好的相关性（y = 1.243x + 3.61，r = 0.904）。将AV6B方法（y）的值与PRM方法（x）的值进行比较时，相关性很低（y = 1.406x-29.15，r = 0.786）。总之，对于低水平的尿蛋白，AV6B方法比CBB和PRM方法更有用。 J.临床实验室肛门17：147-154，2003年。©2003 Wiley-Liss，Inc.

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