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Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products

机译：多重PCR用于同时鉴定鱼或鱼产品中最常见的欧洲阿片类和杂类

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摘要

Among others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU project. Due to the small size of the metacercariae, the infective stage for human, these parasites cannot be detected visually in fish and monitoring requires expert application of time-consuming techniques. The aim of this was to develop a rapid and affordable molecular method based on multiplex PCR for simultaneous identification of metacercariae of the most common European Opisthorchiid and Heterophyid in fish or fish products.

机译：除其他外，Opithhorchiidae和Heterophyidae家族包括引起鱼源性人畜共患病的多个属，并且也分布在欧洲国家中，并包括在ParaFishControl（欧洲养殖鱼类寄生虫控制的先进工具和研究策略）H2020 EU项目中。由于the尾e的大小很小，是人类的感染阶段，因此无法在鱼类中目测到这些寄生虫，因此监测需要专家应用费时的技术。这样做的目的是开发一种基于多重PCR的快速且负担得起的分子方法，用于同时鉴定鱼或鱼产品中最常见的欧洲阿皮睾丸和杂菌的尾cer。

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期刊名称 Food and Waterborne Parasitology
作者
Monica Caffara; Andrea Gustinelli; Angelica Mazzone; Maria L. Fioravanti;
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作者单位

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年(卷),期 2020(19),-1
年度 2020
页码 -1
总页数 5
原文格式 PDF
正文语种
中图分类生化遗传学;生化药理学;
关键词
Multiplex PCR; Opisthorchiidae; Heterophyidae; Freshwater fish; Fish products; Europe;

机译：多重PCR;甲hor科;H科;淡水鱼;鱼制品;欧洲;

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专利

1. Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products [J] . Monica Caffara, Andrea Gustinelli, Angelica Mazzone, Food and Waterborne Parasitology . 2020,第4期

机译：多重PCR同时鉴定鱼类或鱼类产品中最常见的欧洲Opisthiid和杂合金
2. Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto {Aphia minuta), and Icefish in fresh, marinated and cooked products [J] . Andrea Armani, Lorenzo Castigliego, Lara Tinacci, Food Chemistry . 2012,第1期

机译：多重常规和实时PCR用于鉴定新鲜，腌制和煮熟产品中的Bianchetto（Sardina pilchardus的幼体形式），Rossetto（Aphia minuta）和冰鱼的鱼类
3. Multiplex PCR method for use in real-time PCR for identification of fish fillets from grouper (Epinephelus and Mycteroperca species) and common substitute species. [J] . Trotta M, Schonhuth S, Pepe T, Journal of Agricultural and Food Chemistry . 2005,第6期

机译：用于实时PCR的多重PCR方法，用于鉴定石斑鱼（石斑鱼和Mycteroperca种）和常见替代种的鱼片。
4. A MULTIPLEX-PCR METHOD FOR USE IN REAL-TIME PCR FOR IDENTIFICATION OF FISH FILLETS FROM GROUPER (EPINEPHELUS SPP. AND MYCTEROPERCA SPP.) AND ITS USUAL SUBSTITUTION SPECIES [C] . M. Trotta, S. Schonhuth, T. Pepe, International Conference of the European Aquaculture Society . 2004

机译：用于实时PCR的多重PCR方法，用于鉴定石斑鱼（Epinephelus SPP和MycteroperCA SPP）和通常的替代物种的鱼片
5. Use of PCR Cloning Combined with DNA Barcoding to Identify Fish in a Mixed-Species Product [D] . Silva, Anthony J. 2019

机译：使用PCR克隆与DNA条形码联合用于识别混合物种产品中的鱼类
6. Detection of hemolytic strains of Aeromonas hydrophila and A. sobria along with other Aeromonas spp. from fish and fishery products by multiplex PCR [O] . I. A. Hussain, G. Jeyasekaran, R. Jeya Shakila, 2014

机译：检测嗜水气单胞菌和A. sobria的溶血菌株以及其他气单胞菌属。多重PCR从鱼和鱼产品中提取
7. Molecular identification of Pseudoplatystoma sp. fish fillets by Multiplex PCR / Identificação molecular de filés de peixe Pseudoplatystoma sp. por PCR-Multiplex [O] . Cátia Maria de Oliveira Lobo, Fábio Porto-Foresti, Fernanda Dotti do Prado, 2014

机译：假性乳头状瘤的分子鉴定多重pCR /Identificação分子de filix de peixe pseudoplatystoma sp。通过pCR多重化
8. Ctyptic Translocation Identification in Human and Mouse using Several Telomeric Multiplex FISH (TM-FISH) Strategies [R] . Henegariu, O., Artan, S., Greally, J. M., 2003

机译：使用几种端粒多重FIsH（Tm-FIsH）策略在人和小鼠中进行Ctyptic易位鉴定

Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products

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