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Barcoding of the Genus Culicoides (Diptera: Ceratopogonidae) in Austria—An Update of the Species Inventory Including the First Records of Three Species in Austria

机译:奥地利库里科属的条码编码(双翅目:Ceratopogonidae)—物种清单的更新包括奥地利三种物种的第一个记录

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摘要

Ceratopogonidae are small nematoceran Diptera with a worldwide distribution, consisting of more than 5400 described species, divided into 125 genera. The genus is known to comprise hematophagous vectors of medical and veterinary importance. Diseases transmitted by spp. Such as African horse sickness virus, Bluetongue virus, equine encephalitis virus (Reoviridae) and Schmallenberg virus (Bunyaviridae) affect large parts of Europe and are strongly linked to the spread and abundance of its vectors. However, surveillance measures are not implemented regularly nor in the whole of Austria. In this study, 142 morphologically identified individuals were chosen for molecular analyses (barcoding) of the mitochondrial cytochrome c oxidase subunit I gene (mt COI). Molecular analyses mostly supported previous morphologic identification. Mismatches between results of molecular and morphologic analysis revealed three new species in Austria, Mirzaeva, 1984, which is a member of the Obsoletus group, Kieffer, 1918 and Kieffer, 1919 as well as possible cryptic species. We present here the first Austrian barcodes of the mt COI region of 26 species and conclude that barcoding is a reliable tool with which to support morphologic analysis, especially with regard to the difficult to identify females of the medically and economically important genus .
机译:Ceratopogonidae是小型线虫双足纲动物,分布在世界各地,由5400多个上述物种组成,分为125属。已知该属包括具有医学和兽医学重要性的食血载体。 spp传播的疾病。例如非洲的马病病毒,蓝舌病病毒,马脑​​炎病毒(Reoviridae)和Schmallenberg病毒(Bunyaviridae)会影响欧洲大部分地区,并与其载体的传播和丰富程度密切相关。但是,没有定期或在整个奥地利实施监视措施。在这项研究中,选择了142个在形态学上已鉴定的个体用于线粒体细胞色素C氧化酶亚基I基因(mt COI)的分子分析(条形码)。分子分析主要支持先前的形态学鉴定。分子和形态分析结果之间的不匹配揭示了奥地利的三个新种, 米尔扎耶娃(1984年)是Obsoletus小组的成员,基弗(1918)和基弗(1919)以及可能的神秘物种。我们在此介绍26种mt COI区域的第一个奥地利条形码,并得出结论,条形码是一种可靠的工具,可用来支持形态学分析,尤其是对于难以识别具有医学和经济意义的属的雌性而言。

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