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首页> 美国卫生研究院文献>The Journal of Clinical Investigation >Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro.
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Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro.

机译：人内皮细胞-淋巴细胞相互作用。内皮细胞起体外促分裂原诱导的人类T淋巴细胞活化所必需的辅助细胞的作用。

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Mitogen-stimulated human T cell activation is absolutely dependent on the participation of a nonresponding accessory cell. In populations of human peripheral blood mononuclear cells, monocytes function as the requisite accessory cells. The possibility that cultured endothelial cells (EC) might also function as accessory cells was studied by examining the potential of endothelial cells to restore mitogen responsiveness to monocyte-depleted human T cells. Highly purified T cells were prepared by isolating cells rosetting with sheep erythrocytes and removing monocyte contamination by glass adherence and nylon wool column passage. When cultured at low cell density, T cells failed to respond to stimulation with various mitogenic lectins, whereas co-culture with monocytes restored responsiveness. Similarly, EC obtained from umbilical vein, pulmonary artery, and ovarian vein restored the capacity of T cells to respond to mitogens. Mitogen-stimulated T cell activation required viable endothelial cells. Moreover, effective endothelial T cell cooperation appeared to involve the establishment of cell-to-cell contact between EC and responding T cells. Accessory cell function was not a nonspecific property of all tissue culture cells as evidenced by the finding that human foreskin fibroblasts, lung fibroblasts, and HeLa cells were unable to restore responsiveness to monocyte-depleted T cells. These observations indicate that endothelial cells can support the induction of mitogen-induced T cell activation and suggest that cells lining blood vessels may play an active role in the initiation of immune responses in vivo.
机译：丝裂原刺激的人类T细胞活化绝对依赖于无反应的辅助细胞的参与。在人类外周血单核细胞群体中，单核细胞充当必需的辅助细胞。通过检查内皮细胞恢复有丝分裂原对单核细胞减少的人T细胞的反应能力的可能性，研究了培养的内皮细胞（EC）也可能作为辅助细胞的可能性。通过分离与绵羊红细胞成玫瑰花样的细胞，并通过玻璃粘附和尼龙毛柱分离除去单核细胞污染，从而制备高纯度的T细胞。当以低细胞密度培养时，T细胞无法对各种促有丝分裂凝集素的刺激做出反应，而与单核细胞共培养则恢复了反应能力。同样，从脐静脉，肺动脉和卵巢静脉获得的EC恢复了T细胞对有丝分裂原作出反应的能力。丝裂原刺激的T细胞活化需要有活力的内皮细胞。此外，有效的内皮T细胞合作似乎涉及在EC和响应性T细胞之间建立细胞间接触。辅助细胞功能并非所有组织培养细胞的非特异性，这是由以下发现所证实的：人的包皮成纤维细胞，肺成纤维细胞和HeLa细胞无法恢复对单核细胞减少的T细胞的反应能力。这些观察结果表明，内皮细胞可以支持诱导丝裂原诱导的T细胞活化，并且表明衬里血管的细胞可以在体内免疫应答的起始中发挥积极作用。

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