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首页> 美国卫生研究院文献>Nature Public Health Emergency Collection >Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease
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Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease

机译：犬细小病毒疫苗株的部分DNA克隆和测序：核酸杂交在犬细小病毒病诊断中的应用

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摘要

The cloning and sequencing of an RI- I fragment derived from the replicative form of a canine parvovirus (CPV) vaccine strain are reported. The variability of the 5′ end of NS 1 protein gene in the genome is confirmed by comparison with previously determined DNA sequences. A 15 nucleotide deletion was also observed in this vaccine strain. In order to improve CPV diagnosis, radioactively labelled RNA or DNA and biotin labelled DNA obtained by random priming of the recombinant plasmid were used as probes mainly on gut or stool samples from naturally infected dogs. Results of filter hybridization correlated well with histopathological diagnosis of parvovirus infection and with hemagglutination tests performed on dog faeces. We propose that nucleic acid hybridization may be an alternative diagnostic method to ascertain the presence of CPV, especially in frozen samples.
机译：已经报道了源自犬细小病毒（CPV）疫苗株的复制形式的RI-1片段的克隆和测序。通过与先前确定的DNA序列比较，证实了基因组中NS 1蛋白基因的5'端的变异性。在该疫苗株中也观察到15个核苷酸的缺失。为了改善CPV诊断，通过随机引发重组质粒获得的放射性标记的RNA或DNA和生物素标记的DNA主要用作天然感染狗的肠或粪便样品的探针。滤膜杂交的结果与细小病毒感染的组织病理学诊断以及对狗粪便进行的血凝试验密切相关。我们建议核酸杂交可能是确定CPV存在的另一种诊断方法，尤其是在冷冻样品中。

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