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首页> 美国卫生研究院文献>The Journal of Clinical Investigation >Molecular cloning and characterization of human cardiac alpha- and beta-form myosin heavy chain complementary DNA clones. Regulation of expression during development and pressure overload in human atrium.
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Molecular cloning and characterization of human cardiac alpha- and beta-form myosin heavy chain complementary DNA clones. Regulation of expression during development and pressure overload in human atrium.

机译：人类心脏α和β型肌球蛋白重链互补DNA克隆的分子克隆和表征。发育过程中的表达调节和人心房压力超负荷。

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We have constructed and characterized two types of myosin heavy chain (MHC) cDNA clones (pHMHC2, pHMHC5) from a fetal human heart cDNA library. Comparison of the nucleotide and deduced amino acid sequences between pHMHC2 and pHMHC5 shows 95.1 and 96.2% homology, respectively. The carboxyl-terminal peptide and 3'-untranslated (3'-UT) regions are highly divergent and specific for these cDNA clones. By using the synthetic oligonucleotide probes that are complementary to the unique 3'-UT regions of these cDNA clones, we demonstrate that pHMHC2 is exclusively transcribed in the atrium, whereas the mRNA for pHMHC5 is predominantly expressed in the ventricle. This result indicates that pHMHC2 and pHMHC5 code for alpha- and beta-form MHCs, respectively. Furthermore, we show that beta-form MHC mRNA is expressed in adult atrium at a low level but scarcely expressed in fetal atrium. Finally, we demonstrate that MHC isozymic transition in pressure-overloaded atrium is, at least in part, regulated at a pretranslational level.
机译：我们已经从胎儿的人心脏cDNA文库构建并鉴定了两种类型的肌球蛋白重链（MHC）cDNA克隆（pHMHC2，pHMHC5）。 pHMHC2和pHMHC5之间核苷酸和推导氨基酸序列的比较分别显示95.1％和96.2％的同源性。羧基末端肽和3'-非翻译（3'-UT）区高度分歧，并且对这些cDNA克隆具有特异性。通过使用与这些cDNA克隆的独特3'-UT区互补的合成寡核苷酸探针，我们证明了pHMHC2仅在心房中转录，而pHMHC5的mRNA主要在心室中表达。该结果表明pHMHC2和pHMHC5分别编码α型和β型MHC。此外，我们表明，β型MHC mRNA在成人心房中低水平表达，而在胎儿心房中很少表达。最后，我们证明压力超负荷心房中的MHC同工酶转变至少部分受翻译前水平的调节。

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