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首页> 美国卫生研究院文献>Microorganisms >Characterization of the Phenanthrene-Degrading Sphingobium yanoikuyae SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway
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Characterization of the Phenanthrene-Degrading Sphingobium yanoikuyae SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway

机译：重金属共存液体介质中菲降解性天竺葵鞘氨醇SJTF8的表征及代谢途径分析

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Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, SJTF8 was isolated and identified with great and stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, and anthracene) and heterocyclic and halogenated aromatic compounds (dibenzothiophene and 9-bromophenanthrene) as the sole carbon source. It could degrade over 98% of 500 mg/L phenanthrene in 4 days, and the -3,4-dihydrophenanthrene-3,4-diol was the first-step intermediate. Notably, strain SJTF8 showed great tolerance to heavy metals and acidic pH. Supplements of 0.30 mM of Cu , 1.15 mM of Zn , and 0.01 mM of Cd had little effect on its cell growth and phenanthrene degradation; phenanthrene of 250 mg/L could still be degraded completely in 48 h. Further, the whole genome sequence of SJTF8 was obtained, and three plasmids were found. The potential genes participating in stress-tolerance and PAH-degradation were annotated and were found mostly distributed in plasmids 1 and 2. Elimination of plasmid 2 resulted in the loss of the PAH-degradation ability. On the basis of genome mining results, the possible degrading pathway and the metabolites of SJTF8 to phenanthrene were predicted.
机译：多环芳烃（PAH）是常见的有机污染物，具有极大的致癌性，金属/ PAH污染的环境是最困难的补救挑战之一。在这项工作中，即使在压力条件下，SJTF8的分离和鉴定仍具有很高且稳定的PAH降解效率。它可以利用典型的PAH（萘，菲和蒽）以及杂环和卤代芳族化合物（二苯并噻吩和9-溴菲）作为唯一碳源。它可以在4天之内降解超过98％的500 mg / L菲，-3,4-二氢菲-3,4-二醇是第一步中间体。值得注意的是，菌株SJTF8对重金属和酸性pH表现出极大的耐受性。补充0.30 mM的铜，1.15 mM的锌和0.01 mM的镉对其细胞生长和菲降解几乎没有影响。 250 mg / L的菲仍可在48小时内完全降解。此外，获得了SJTF8的全基因组序列，并且发现了三个质粒。注释了参与胁迫耐受性和PAH降解的潜在基因，发现它们主要分布在质粒1和2中。消除质粒2导致PAH降解能力丧失。根据基因组挖掘结果，预测了SJTF8降解为菲的可能途径和代谢产物。

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