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首页> 美国卫生研究院文献>Toxics >Combining Different In Vitro Bioassays to Evaluate Genotoxicity of Water-Accommodated Fractions from Petroleum Products
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Combining Different In Vitro Bioassays to Evaluate Genotoxicity of Water-Accommodated Fractions from Petroleum Products

机译：结合不同的体外生物测定来评估石油产品中水可溶部分的遗传毒性

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摘要

Genotoxicity assessment is of high relevance for crude and refined petroleum products, since oil compounds are known to cause DNA damage with severe consequences for aquatic biota as demonstrated in long-term monitoring studies. This study aimed at the optimization and evaluation of small-scale higher-throughput assays (Ames fluctuation, micronucleus, Nrf2-CALUX ) covering different mechanistic endpoints as first screening tools for genotoxicity assessment of oils. Cells were exposed to native and chemically dispersed water-accommodated fractions (WAFs) of three oil types varying in their processing degree. Independent of an exogenous metabolic activation system, WAF compounds induced neither base exchange nor frame shift mutations in bacterial strains. However, significantly increased chromosomal aberrations in zebrafish liver (ZF-L) cells were observed. Oxidative stress was indicated for some treatments and was not correlated with observed DNA damage. Application of a chemical dispersant increased the genotoxic potential rather by the increased bioavailability of dissolved and particulate oil compounds. Nonetheless, the dispersant induced a clear oxidative stress response, indicating a relevance for general toxic stress. Results showed that the combination of different in vitro assays is important for a reliable genotoxicity assessment. Especially, the ZF-L capable of active metabolism and DNA repair seems to be a promising model for WAF testing.
机译：遗传毒性评估与原油和精炼石油产品具有高度相关性，因为长期监测研究表明，已知油化合物会导致DNA破坏，并对水生生物造成严重后果。这项研究旨在优化和评估涵盖不同机制终点的小规模高通量分析（Ames波动，微核，Nrf2-CALUX），作为油类遗传毒性评估的第一个筛选工具。将细胞暴露于加工程度不同的三种油类型的天然分散液和化学分散的水浓缩级分（WAF）。与外源性代谢激活系统无关，WAF化合物既不诱导细菌菌株中的碱基交换突变也不引起移码突变。但是，观察到斑马鱼肝脏（ZF-L）细胞中的染色体畸变显着增加。某些治疗表明存在氧化应激，与观察到的DNA损伤无关。化学分散剂的使用增加了遗传毒性潜力，而不是通过增加溶解的和颗粒状油化合物的生物利用度来提高。然而，分散剂引起明显的氧化应激反应，表明与一般毒性应激有关。结果表明，不同的体外测定方法的组合对于可靠的遗传毒性评估很重要。特别是，具有活性代谢和DNA修复能力的ZF-L似乎是WAF测试的有希望的模型。

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