• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Clinical Investigation >Expression of two immediate early genes Egr-1 and c-fos in response to renal ischemia and during compensatory renal hypertrophy in mice.
【2h】

Expression of two immediate early genes Egr-1 and c-fos in response to renal ischemia and during compensatory renal hypertrophy in mice.

机译：响应肾脏缺血和小鼠代偿性肾肥大期间两个立即早期基因Egr-1和c-fos的表达。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To identify specific genetic regulatory mechanisms associated with renal ischemia, we measured the accumulation of Egr-1 and c-fos mRNAs in the mouse kidney after occlusion of the renal artery and reperfusion. At 1 h after right nephrectomy and arterial occlusion of the contralateral kidney for 10 or 30 min, Egr-1 mRNA levels were three to five times greater in these kidneys as compared with those in control animals that had sustained unilateral nephrectomy alone and were much greater than levels in the normal organ. Whether ischemia was imposed for 10 or for 30 min, renal Egr-1 mRNA contents were equivalent and remained elevated after 24 h of reperfusion subsequent to 30 min of ischemia. Although c-fos mRNA also accumulated in response to ischemia and reperfusion, the pattern differed from that of Egr-1 in that c-fos mRNA content varied with the duration of ischemia and was undetectable 24 h after injury. Contralateral nephrectomy was not necessary to see the marked accumulation of Egr-1 and c-fos mRNAs with unilateral ischemia. Reflow was necessary, however, since only minimal sequence accumulation occurred by the end of the ischemic period. After left uninephrectomy alone, Egr-1 mRNA levels in the remaining kidney were maximal 30 min after surgery, but were not detectable thereafter; c-fos mRNA levels did not change after unilateral nephrectomy. Differential expression of early growth-related genes implicated in transcriptional activation may influence tissue recovery after renal ischemia.
机译：为了确定与肾脏缺血相关的特定遗传调控机制，我们测量了肾动脉闭塞和再灌注后小鼠肾脏中Egr-1和c-fos mRNA的积累。右肾切除术和对侧肾脏动脉闭塞10或30分钟后1小时，这些肾中的Egr-1 mRNA水平比仅进行单侧肾切除术的对照组动物高3至5倍，并且更大超过正常器官的水平。无论是实施缺血10分钟还是进行30分钟，缺血30分钟后再灌注24小时后，肾脏Egr-1 mRNA的含量均相等并保持升高。尽管c-fos mRNA也随缺血和再灌注而积累，但其模式与Egr-1有所不同，因为c-fos mRNA的含量随缺血持续时间而变化，并且在损伤后24小时无法检测到。对侧肾切除术不必观察到单侧缺血的Egr-1和c-fos mRNA的显着积累。但是，由于在缺血期结束前仅发生了最小的序列积累，因此有必要进行回流。单独进行左肾切除术后，其余肾脏中Egr-1 mRNA的水平在手术后30分钟达到最大值，但此后无法检测到。单侧肾切除术后c-fos mRNA水平未改变。涉及转录激活的早期生长相关基因的差异表达可能影响肾缺血后的组织恢复。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号