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Changes in the Content of Some Groups of Phenolic Compounds and Biological Activity of Extracts of Various Parts of Heather (Calluna vulgaris (L.) Hull) at Different Growth Stages

机译:一些酚类化合物含量的变化以及Heather各种部位提取物的含量和生物活性(Calluna寻常(L.)船体)在不同的生长阶段

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摘要

Heather ( (L.) Hull.) is noted for a diverse chemical composition and a broad range of biological activity. The current study was aimed at monitoring changes in the accumulation of certain groups of phenolic compounds in various organs of heather (leaves, stems, roots, rhizomes, flowers, and seeds) at different growth stages (vegetative, floral budding, flowering, and seed ripening) as well as studying antioxidant (employing the DPPH and FRAP assays) and antibacterial activity of its extracts. The highest total amount of phenolic compounds, tannins, flavonoids, hydroxycinnamic acids, and proanthocyanidins was detected in leaves and roots at all growth stages, except for the flowering stage. At the flowering stage, the highest content of some groups of phenolic compounds (flavonoids, proanthocyanidins, and anthocyanins) was observed in flowers. Highest antioxidant activity was recorded for the flower extracts (about 500 mg of ascorbic acid equivalents per gram according to the DPPH assay) and for the leaf extract at the ripening stage (about 350 mg of ascorbic acid equivalents per gram according to the FRAP assay). Strong correlation was noted between antioxidant activity (DPPH) and the content of anthocyanins ( = 0.75, ≤ 0.01) as well as between antioxidant activity (FRAP) and the total content of phenolic compounds ( = 0.77, ≤ 0.01). Leaf extracts and stem extracts turned out to perform antibacterial action against both gram-negative and gram-positive bacteria, whereas root extracts appeared to be active only against , and rhizome extracts against .
机译:Heather((L.)Hull。)被指出,用于多种化学成分和广泛的生物活性。目前的研究旨在监测不同生长阶段(叶片,茎,根,根茎,花和种子)各种器官中某些酚类化合物的积累变化(植物,花卉芽,开花和种子成熟)以及研究抗氧化剂(使用DPPH和FRAP测定)和其提取物的抗菌活性。除了开花阶段,在叶片和根中检测到最高总量的酚类化合物,单宁,类黄酮,羟基氨基,羟基琥珀酸和原花青素。在开花阶段,在花中观察到一些酚类化合物(类黄酮,原花青素和花青素)的最高含量。为花提取物(根据DPPH测定的每克约500mg抗坏血酸当量)记录最高的抗氧化活性,并且根据FRAP测定法在成熟阶段的叶子提取物(根据FRAP测定法每克约350mg抗坏血酸当量) 。在抗氧化活性(DPPH)和花青素(= 0.75,≤0.01)以及抗氧化活性(FRAP)之间的含量和酚类化合物的总含量(= 0.77,≤0.01)之间存在强烈的相关性。结果叶提取物和干萃取物原因对革兰氏阴性和革兰氏阳性细菌进行抗菌作用,而根萃取物似乎仅针对活性和根瘤菌提取物。

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