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首页> 美国卫生研究院文献>Molecules >LC–MS-Based Lipidomic Analysis of Serum Samples from Spontaneously Hypertensive Rats Treated with an Extract of Acanthopanax sessiliflorus Fruits
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LC–MS-Based Lipidomic Analysis of Serum Samples from Spontaneously Hypertensive Rats Treated with an Extract of Acanthopanax sessiliflorus Fruits

机译：基于LC-MS的血清样品从Acanthopanax Sessiliflorus果实提取物治疗的自发性高血压大鼠血清样品

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Recently, lipidomics has revealed that many diseases are highly associated with altered lipid metabolism, as in the case of hypertension affecting serum lipid metabolism. In this study, an LC–MS-based lipidomic approach was used to profile serum lipids in spontaneously hypertensive rats (SHRs) treated with an extract of fruits (ASF), to elucidate the serum lipid metabolism alteration by hypertension and the treatment of a drug or ASF. First, UPLC-QTOF/MS profiled a total of 208 lipids from six pooled samples of normal controls, SHR, SHR + 100 mg/kg of drug, and SHR + ASF 200, 400, or 600 mg/kg. These six groups were differentiated by the PCA and sPLS–DA, and 120 lipid species were identified as differentially regulated lipids (DRLs) by ANOVA ( values < 0.05). Second, UPLC–QqQ/MS was used for the target profiling of 120 DRLs from individual samples of the six groups. Using an ANOVA, 67 lipids (38 TGs, 4 DGs, 17 PCs, 2 PEs, and 6 LPCs) were selected as validated DRLs. The mostly altered lipids, such as TG (62:13), TG (60:13), PC (34:4), PC (36:5), and PC (38:2), were decreased in SHR compared to the normal control, and received little by treatment with ASF. These results demonstrated the correlation between hypertension and serum lipid metabolism. Furthermore, both drug and ASF treatment similarly altered the lipid profiles of SHRs. Finally, we found that DRLs have the potential to help us to interpret the lipid metabolism of hypertension.
机译：最近，脂质病揭示了许多疾病与改变的脂质代谢具有高度相关，如在影响血清脂质代谢的高血压的情况下。在该研究中，使用基于LC-MS的脂质族方法在用水果（ASF）提取物处理的自发性高血压大鼠（SHR）中概述血清脂质，以通过高血压和治疗药物来阐明血清脂质代谢改变或asf。首先，UPLC-QTOF / MS通过六个正常对照样品，SHR，SHR + 100mg / kg药物和SHR + ASF 200,400，400或600mg / kg的六个汇集样品，共有208个脂质。通过PCA和SPLS-DA分化这六组，通过ANOVA鉴定为差异调节的脂质（DRL）的120种脂质物种（值<0.05）。其次，UPLC-QQQ / MS用于来自六组的各个样品的120dls的目标分析。使用ANOVA，67脂质（38 TGS，4 DGS，17个，2 PES和6LPC）被选择为验证的DRL。与TG（60:13），PC（36：4），PC（36：5），PC（36：5），PC（36：5），PC（36：5），PC（36：3），PC（36：5），PC（36：5）和PC（38：2）。正常对照，并通过ASF治疗收到少。这些结果表明了高血压和血清脂质代谢之间的相关性。此外，药物和ASF治疗的同样改变了SHR的脂质谱。最后，我们发现DRL有可能帮助我们解释高血压的脂质代谢。

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