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首页> 美国卫生研究院文献>Genes >Dramatic Increase in Content of Diverse Flavonoids Accompanied with Down-Regulation of F-Box Genes in a Chrysanthemum (Chrysanthemum × morifolium (Ramat.) Hemsl.) Mutant Cultivar Producing Dark-Purple Ray Florets
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Dramatic Increase in Content of Diverse Flavonoids Accompanied with Down-Regulation of F-Box Genes in a Chrysanthemum (Chrysanthemum × morifolium (Ramat.) Hemsl.) Mutant Cultivar Producing Dark-Purple Ray Florets

机译：各种黄酮类化合物含量的显着增加伴随着菊花中F型箱基因的下调（Chrysanthemum×Morifolium（Ramat）Hemsl。）突变体栽培品种产生深紫色的小花

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Anthocyanins (a subclass of flavonoids) and flavonoids are crucial determinants of flower color and substances of pharmacological efficacy, respectively, in chrysanthemum. However, metabolic and transcriptomic profiling regarding flavonoid accumulation has not been performed simultaneously, thus the understanding of mechanisms gained has been limited. We performed HPLC-DAD-ESI-MS (high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization mass spectrometry) and transcriptome analyses using “ARTI-Dark Chocolate” (AD), which is a chrysanthemum mutant cultivar producing dark-purple ray florets, and the parental cultivar “Noble Wine” for metabolic characterization and elucidation of the genetic mechanism determining flavonoid content. Among 26 phenolic compounds identified, three cyanidins and eight other flavonoids were detected only in AD. The total amounts of diverse flavonoids were 8.0 to 10.3 times higher in AD. Transcriptome analysis showed that genes in the flavonoid biosynthetic pathway were not up-regulated in AD at the early flower stage, implying that the transcriptional regulation of the pathway did not cause flavonoid accumulation. However, genes encoding post-translational regulation-related proteins, especially F-box genes in the mutated gene, were enriched among down-regulated genes in AD. From the combination of metabolic and transcriptomic data, we suggest that the suppression of post-translational regulation is a possible mechanism for flavonoid accumulation in AD. These results will contribute to research on the regulation and manipulation of flavonoid biosynthesis in chrysanthemum.
机译：花青素（类黄酮的亚类）和黄酮类化合物是菊花中的花色和药理学效能的物质的关键决定因素。然而，关于类黄酮积累的代谢和转录组分布尚未同时进行，因此对所获得的机制的理解受到限制。我们进行了HPLC-DAD-ESI-MS（高性能液相色谱，与光电二极管阵列检测和电喷雾电离质谱法）和使用“ARTI-Dark巧克力”（AD）分析的转录组分析，这是一种产生深紫色的菊花突变体栽培品种雷小花，以及父母品种“贵族葡萄酒”用于代谢表征和阐明确定类黄酮含量的遗传机制。在鉴定的26种酚类化合物中，仅在AD中检测到三种青色素和八种其他黄酮。广告中各种黄酮类化合物的总量为8.0至10.3倍。转录体分析表明，早期花阶段的广告中黄酮类生物合成途径中的基因尚未上调，这意味着途径的转录调节不会引起黄酮类积累。然而，编码翻译后调节相关蛋白质，特别是突变基因中的F型箱基因的基因在AD中的下调基因中富集。根据代谢和转录组数据的组合，我们建议抑制翻译后调节是广告中黄酮类化合物的可能机制。这些结果将有助于研究菊花中黄酮类生物合成的调控和操纵。

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