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Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species Solanum microdontum and Solanum pampasense

机译:从野生马铃薯种类茄子微腔和Solanum Pampasense源自源于野生马铃薯种类的晚期抗血管型QTL

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摘要

Wild potato species continue to be a rich source of genes for resistance to late blight in potato breeding. Whilst many dominant resistance genes from such sources have been characterised and used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the resistance can be identified and tagged using molecular markers. In this study, F populations were created from crosses between blight susceptible parents and lines exhibiting strong partial resistance to late blight derived from the South American wild species and . Both populations exhibited continuous variation for resistance to late blight over multiple field-testing seasons. High density genetic maps were created using single nucleotide polymorphism (SNP) markers, enabling mapping of quantitative trait loci (QTLs) for late blight resistance that were consistently expressed over multiple years in both populations. In the population created with the source, QTLs for resistance consistently expressed over three years and explaining a large portion (21–47%) of the phenotypic variation were found on chromosomes 5 and 6, and a further resistance QTL on chromosome 10, apparently related to foliar development, was discovered in 2016 only. In the population created with the source, QTLs for resistance were found in over two years on chromosomes 11 and 12. For all loci detected consistently across years, the QTLs span known R gene clusters and so they likely represent novel late blight resistance genes. Simple genetic models following the effect of the presence or absence of SNPs associated with consistently effective loci in both populations demonstrated that marker assisted selection (MAS) strategies to introgress and pyramid these loci have potential in resistance breeding strategies.
机译:野马铃薯物种继续成为抗马铃薯育种的抗性抗性的丰富基因来源。虽然来自这种来源的许多显性抗性基因已经表征并用于育种,但是当使用分子标记可以识别和标记电阻的基因座时,定量抗性也提供繁殖潜力。在这项研究中,F群体是从枯萎之间的裂缝和线路之间产生的跨越群体,从而呈现出南美野生物种的强烈部分抗性。这两个群体都表现出对多场测试季节的抵抗抗性的持续变化。使用单核苷酸多态性(SNP)标记产生高密度遗传图,使定量性状的定位映射为晚期枯燥的抗性,这些抗性在两年内持续多年的群体。在用源产生的群体中产生的群体中,在染色体5和6上发现致力于三年的抗抵抗力并解释了表型变异的大部分(21-47%),并且在染色体10上进行了进一步的电阻QTL,显然有关致命的发展,仅在2016年发现了。在用源产生的群体中,在染色体11和12中发现了抗抵抗的QTL。对于跨越多年来一直检测到的所有基因座,QTLS跨越R基因簇,因此它们可能代表新的晚期枯萎基因。在两种群体中持续有效基因座相关的SNP存在或不存在的简单遗传模型证明了标记辅助选择(MAS)初始和金字塔的策略这些基因座具有抵抗育种策略的潜力。

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