A novel rapid technique for clearing leaf tissues

机译：一种用于清除叶片组织的新型快速技术

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摘要

Clearing leaves is a highly useful practice for many taxonomic, ecological, physiological, and eco‐physiological aspects of research. Using traditional methods, the procedure for clearing a leaf (referred to as diaphanization) can take several days or even weeks. In our laboratory we developed a technique, originally used for dissociating wood, that yields excellent epidermal and leaf venation preparations clearly showing the details of epidermal cells, hydathodes, trichomes, leaf margins, and leaf venation, in a maximum of three days.

机译：清算叶是许多分类，生态，生理学和生态生理学方面的高度有用的实践。使用传统方法，清除叶片（称为透明度）的程序可能需要几天甚至几周。在我们的实验室中，我们开发了一种最初用于解离木材的技术，产生优异的表皮和叶静脉制剂，清楚地显示表皮细胞，HadeDodes，毛状体，叶片边缘和叶静脉的细节，最多三天。

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期刊名称 Applications in Plant Sciences
作者
Emmanuel García‐Gutiérrez; Fernando Ortega‐Escalona; Guillermo Angeles;
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作者单位

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年(卷),期 2020(8),9
年度 2020
页码 e11391
总页数 8
原文格式 PDF
正文语种
中图分类
关键词
diaphanization; leaf clearing; leaf epidermis; leaf venation; stomata; trichomes;

机译：透明度;叶片清除;叶片表皮;叶静脉;气孔;毛状体;

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专利

1. A novel, rapid technique for clearing leaf tissues [J] . Emmanuel García‐Gutiérrez, Fernando Ortega‐Escalona, Guillermo Angeles Applications in plant sciences. . 2020,第9期

机译：一种用于清除叶片组织的新型快速技术
2. A rapid and effective optical-clearing technique for deep tissue fluorescence imaging in trees [J] . Trees. Structure and Function . 2020,第3期

机译：树木深组荧光成像的快速有效的光学清算技术
3. A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue [J] . Azaripour Adriano, Lagerweij Tonny, Scharfbillig Christina, Progress in Histochemistry and Cytochemistry . 2016,第2期

机译：专门针对结缔组织的组织3D成像清除技术概述
4. Rapid Stokes imaging applied to monitor oscillations during optical tissue clearing [C] . Jiawei Song, Nan Zeng, Hui Ma Conference on Polarized Light and Optical Angular Momentum for Biomedical Diagnostics . 2021

机译：快速斯托克斯成像应用于在光学组织清除期间监测振荡
5. Titanium Micromachining Process Advancements for Optical Tissue Clearing [D] . Corber, Samantha R. 2020

机译：光学组织清除的钛微机械加工进程
6. Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues [O] . Dana M. Freund, Katherine A. Sammons, Nokwanda P. Makunga, 2018

机译：叶片喷雾质谱：快速环境电离技术可直接评估植物组织代谢物
7. A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue [O] . Azaripour, Adriano, Lagerweij, Tonny, Scharfbillig, Christina, 2016

机译：专门针对结缔组织的组织3D成像清除技术概述

A novel rapid technique for clearing leaf tissues

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