• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Antioxidants >Protective Effect of
【2h】

Protective Effect of

机译：保护效果

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pyrus ussuriensis Maxim (Korean pear) has been used for hundreds of years as a traditional herbal medicine for asthma, cough, and atopic dermatitis in Korea and China. Although it was originally shown to possess anti-inflammatory, antioxidant, and antiatopic properties, its gastroprotective effects have not been investigated. In the present study, we evaluated the protective effects of Pyrus ussuriensis Maxim extract (PUE) against ethanol-induced gastritis in rats. The bioactive compound profile of PUE was determined by gas chromatography mass spectroscopy (GC-MS) and high-performance liquid chromatography (HPLC). The gastroprotection of PUE at different doses (250 and 500 mg/kg body weight) prior to ethanol ingestion was evaluated using an in vivo gastritis rat model. Several endpoints were evaluated, including gastric mucosal lesions, cellular degeneration, intracellular damage, and immunohistochemical localization of leucocyte common antigen. The gastric mucosal injury and ulcer score were determined by evaluating the inflamed gastric mucosa and by histological examination. To identify the mechanisms of gastroprotection by PUE, antisecretory action and plasma prostaglandin E2 (PGE2), gastric mucosal cyclic adenosine monophosphate (cAMP), and histamine levels were measured. PUE exhibited significant antioxidant effects with IC50 values of 56.18 and 22.49 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition (%), respectively. In addition, GC/MS and HPLC analyses revealed several bioactive compounds of PUE. Pretreatment with PUE significantly (p < 0.05) decreased the ulcer index by preventing gastric mucosal lesions, erosion, and cellular degeneration. An immunohistochemical analysis revealed that PUE markedly attenuated leucocyte infiltration in a dose-dependent manner. The enhancement of PGE2 levels and attenuation of cAMP levels along with the inhibition of histamine release following PUE pretreatment was associated with the cytoprotective and healing effects of PUE. In contrast, the downregulation of the H+/K+ ATPase pathway as well as muscarinic receptor (M3R) and histamine receptor (H2R) inhibition was also involved in the gastroprotective effects of PUE; however, the expression of cholecystokinin-2 receptors (CCK2R) was unchanged. Finally, no signs of toxicity were observed following PUE treatment. Based on our results, we conclude that PUE represents an effective therapeutic option to reduce the risk of gastritis and warrants further study.
机译：Pyrus Ussuriensis Maxim（韩国梨）已被用于韩国和中国的传统草药作为传统的草药，韩国和中国的特应性皮炎。虽然最初显示出具有抗炎，抗氧化剂和防脱石性质，但尚未研究其胃保护作用。在本研究中，我们评估了Pyrus Ussuriensis Maxim提取物（PUE）对大鼠乙醇诱导的胃炎的保护作用。通过气相色谱质谱（GC-MS）和高性能液相色谱（HPLC）测定耦合的生物活性化合物曲线。在体内胃炎大鼠模型中评估在乙醇摄入前的不同剂量（250和500mg / kg体重）的紫外线的胃开胃解光保护。评估几个终点，包括胃粘膜病变，细胞变性，细胞内损伤和白细胞常见抗原的免疫组化定位。通过评估发炎的胃粘膜和组织学检查来确定胃粘膜损伤和溃疡评分。为了鉴定Pue，防盗组织和血浆前列腺素E2（PGE2），测定胃黏膜环状腺苷一磷酸（CAMP）和组胺水平的胃阳离子。 Pue表现出具有56.18和22.49μg/ ml的IC 50值的显着抗氧化作用，对于2,2-二苯基-1-富铬酰基（DPPH）和2,2'-β-二甲基唑啉）-6-磺酸（ABTS） ）分别抑制（％）。此外，GC / MS和HPLC分析揭示了几种灌花的生物活性化合物。用预处理（P <0.05）预处理（P <0.05）通过防止胃粘膜病变，腐蚀和细胞变性降低了溃疡指数。免疫组织化学分析表明，扁平以剂量依赖性方式显着衰减白细胞浸润。 Pue2水平的增强以及CAMP水平的衰减随着扁平预处理后组胺释放的抑制与扁平的细胞保护和愈合作用有关。相反，H + / K + ATP酶途径以及毒蕈碱受体（M3R）和组胺受体（H2R）抑制的下调也参与了扁平的胃保护作用;然而，胆囊蛋白-2受体（CCK2R）的表达不变。最后，在颊施后没有观察到毒性的迹象。根据我们的结果，我们得出结论，PUE代表了降低胃炎风险的有效治疗选择，并认证进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号