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Adipose and Muscle Cell Co-Culture System: A Novel In Vitro Tool to Mimic the In Vivo Cellular Environment

机译：脂肪和肌肉细胞共同培养系统：模拟体内细胞环境的一种新型体外工具

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摘要

Co-culture system provides a novel platform to study interaction between different cell types in an in-vitro method. The co-cultures techniques have played key role in the understanding of cell–cell communication and relevant for drug response analysis. Co-culture system could influence therapeutic drug response in a dose dependent manner which reflects the clinical situation in patients. Also, the co-culture system may reflect a more realistic environment that similar phenotypic and functional characteristics of in vivo conditions. We also suggest that the co-culture methods as a key tool to study the interaction between adipose and muscle tissue under various environments including drug responses, production and influence of secretory factors, cell growth, and development. Therefore, the co-cultures method has been shown wide applications in cell biology study.

机译：共同培养系统提供了一种新颖的平台，用于研究不同细胞类型在体外方法中的相互作用。共培养技术在对细胞 - 细胞通信的理解中发挥了关键作用，并对药物反应分析相关。共培养系统可以以剂量依赖方式影响治疗药物反应，反映患者的临床情况。而且，共培养系统可以反映更现实的环境，类似于体内条件的类似表型和功能特征。我们还建议，共同文化方法作为研究各种环境下脂肪和肌肉组织之间相互作用的关键工具，包括药物反应，生产和分泌因子，细胞生长和发育。因此，在细胞生物学研究中已经显示了共培养方法广泛的应用。

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期刊名称 Biology
作者
Palaniselvam Kuppusamy; Dahye Kim; Ilavenil Soundharrajan; Inho Hwang; Ki Choon Choi;
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作者单位

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年(卷),期 2021(10),1
年度 2021
页码 6
总页数 12
原文格式 PDF
正文语种
中图分类生物学;
关键词
co-culture; in vitro technique; adipocytes; muscle; secretory factors;

机译：共同培养;体外技术;脂肪细胞;肌肉;分泌因子;

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专利

1. In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes [J] . Nobuyoshi Shimizu, Tetsuhiko Yoshida, Yoshitaka Yamaguchi, Biology Open . 2014,第11期

机译：体外共培养系统研究表达Aire的髓样胸腺上皮细胞与新鲜胸腺细胞之间的细胞相互作用的分子基础体外共培养系统研究表达Aire的髓样胸腺上皮细胞与新鲜胸腺细胞之间的细胞相互作用的分子基础共培养系统，用于研究表达Aire的髓样胸腺上皮细胞与新鲜胸腺细胞之间的细胞相互作用的分子基础
2. In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes [J] . Jun Kudoh, Nobuyoshi Shimizu, Tetsuhiko Yoshida, Biology Open . 2014,第11期

机译：研究表达Aire的髓样胸腺上皮细胞与新鲜胸腺细胞之间细胞相互作用的分子基础的体外共培养系统
3. Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo [J] . Yunfeng Lin, En Luo, Xizhe Chen, Journal of cellular and molecular medicine. . 2005,第4期

机译：脂肪组织来源的基质细胞体外成软骨分化和体内软骨形成过程中的分子和细胞表征
4. THE REPETITIVE FREEZE-THAW- IN VIVO AND IN VITRO SUBCULTURE METHOD (RFTIVIVSCM) TO INDEFINITELY PRESERVE HUMAN BONE MARROW AND ADIPOSE TISSUE DERIVED MESENCHYMAL STROMAL CELLS (MSCs) FOR REGENERATIVE MEDICINE [C] . SUMIDA S., KITAMURA T., MOTOMURA N., IIR workshop on cold applications in life sciences . 2016

机译：体内反复冻融解冻法（RFTIVIVSCM）无限期保留人骨髓和脂肪组织来源的间充质基质细胞（MSCs）作为再生药物
5. Estrogen regulation of adiposity: In vivo and in vitro effects on adipose tissue and muscle metabolism. [D] . D'Eon, Tara Michelle. 2006

机译：雌激素对脂肪的调节：体内和体外对脂肪组织和肌肉代谢的影响。
6. In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes [O] . Yoshitaka Yamaguchi, Jun Kudoh, Tetsuhiko Yoshida, 2014

机译：研究表达Aire的髓样胸腺上皮细胞与新鲜胸腺细胞之间细胞相互作用的分子基础的体外共培养系统
7. Adipose and Muscle Cell Co-Culture System: A Novel In Vitro Tool to Mimic the In Vivo Cellular Environment [O] . Palaniselvam Kuppusamy, Dahye Kim, Ilavenil Soundharrajan, 2020

机译：脂肪和肌肉细胞共同培养系统：模拟体内细胞环境的一种新型体外工具
8. Implantation of In Vitro Tissue Engineered Muscle Repair Constructs and Bladder Acellular Matrices Partially Restore In Vivo Skeletal Muscle Function in a Rat Model of Volumetric Muscle Loss Injury. [R] . Corona, B. T., Ward, C. L., Baker, H. B., 2014

机译：植入体外组织工程肌肉修复构建和膀胱脱细胞基质部分恢复大鼠体积肌肉损伤模型的体内骨骼肌功能。

Adipose and Muscle Cell Co-Culture System: A Novel In Vitro Tool to Mimic the In Vivo Cellular Environment

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