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首页> 美国卫生研究院文献>BMC Biology >Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube
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Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube

机译：Notch Signaling是屋顶板形成的关键引发剂其通过使用背部神经管的RNA分析揭示

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Transcriptome analysis of small groups of premigratory NC and RP cells. a Principal component analysis shows a distinct segregation of transcripts for the purified populations of NC and RP cells along 2 principal components axes (PC1, PC2). b MA plot comparing RP over NC genes. The plot visualizes the differences between measurements taken in two samples, by transforming the data onto M (log ratio) and A (mean average) scales. Genes upregulated in RP when compared to NC are above the red line and downregulated genes are below. c Heat map of 2171 differentially expressed genes (padj< 0.05) between NC and RP. Red and blue colors represent all significantly upregulated and downregulated genes, respectively. Normalized expression is shown after scaling the values for each gene. Genes and samples are ordered by hierarchical clustering, as indicated by the respective dendrograms. d, e Ingenuity pathway analysis (IPA) showing selected enriched categories in RP with reference to NC (red) or downregulated in RP/NC (blue), that include molecular functions and diseases (p value < 0.05). Categories with IPA Z-scores greater than 2 or smaller than − 2 can be considered to be significantly predicted to be up- (d, red) or down- (e, blue) regulated, respectively. f–h Bromodeoxyuridine (BrdU) incorporation following a 1-h pulse at NC (E2, f) or RP (E3.5, g) stages. Note the presence of the BrdU+ nuclei (red) in NC domain but not in RP (delimited by dashed lines). Quantification of Brdu-positive cells (H) shows significantly reduced number of proliferating cells in the RP (*p < 0.001, N = 5/stage). i, j ISH for MycN shows downregulation in RP (delimited by dashed lines). Abbreviations, DRG, dorsal root ganglion, NT, neural tube. Bar = 50 μm
机译：小组预流量NC和RP细胞的转录组分析。主要成分分析显示了沿2个主成分轴（PC1，PC2）的Nc和RP细胞的纯化群体的显着偏析转录物。 B MA Plot比较RP对NC基因的影响。该图通过将数据转换到M（均值平均值）尺度来可视化两个样本中拍摄的测量之间的差异。与NC相比，在RP中上调的基因高于红线，下调基因在下方。 C在Nc和Rp之间的2171次差异表达基因（PADJ <0.05）的C热图。红色和蓝色分别代表所有显着上调和下调的基因。在缩放每个基因的值后显示归一化表达。基因和样品通过分层聚类命令，如各个树枝图所示。 D，e Ingenuey途径分析（IPA）在RP（红色）中显示RP中选择的富集类别，或者在RP / NC（蓝色）中下调，包括分子官能和疾病（P值<0.05）。可以考虑具有大于2或小于-2大于2或小于-2的IPA Z分数的类别，显着预测分别上升（d，红色）或下（e，蓝色）调节。在Nc（E2，F）或RP（E3.5，G）阶段的1-H脉冲之后，F-H溴二氧基脲酰胺（BrdU）掺入。注意NC域中的Brdu +核（红色），但不在RP中（由虚线限制）。 Brdu阳性细胞（H）的定量显示RP中的增殖细胞数显着减少（* P <0.001，n = 5 /阶段）。 I，J ISH for MyCN在RP中显示下调（由虚线分隔）。缩写，DRG，背根神经节，NT，神经管。酒吧=50μm

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