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首页> 美国卫生研究院文献>Cell Transplantation >Impact of One Versus Two Consecutive Doses of Endothelial Cells (EPCs) and EPCs-Derived Condition Medium on Protecting Myocardium from Acute Ischemia-Reperfusion Injury in Rat
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Impact of One Versus Two Consecutive Doses of Endothelial Cells (EPCs) and EPCs-Derived Condition Medium on Protecting Myocardium from Acute Ischemia-Reperfusion Injury in Rat

机译：一种与两种连续剂量的内皮细胞（EPC）和EPCS衍生病症培养基对大鼠急性缺血再灌注损伤保护心肌的影响

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This study tested the impact of single dose and two doses of endothelial progenitor cells (EPCs) and EPCs-derived condition medium (CM) on protecting the left-ventricular myocardium (LVM) from acute ischemia-reperfusion (IR) injury. In vitro study showed EPCs and CM had comparably higher capacity for enhancement of angiogenesis as compared with the controls (all P < .001). Adult-male SD rats (n = 36) were equally categorized into groups 1 (sham-operated control), 2 (IR+vehicle), 3 [IR+EPCs/1.2 × 106/intravenous administration at 3 h after IR procedure), 4 (IR+EPCs/1.2 × 106/at 3 h/24 h after IR), 5 (IR+CM/3.0cc/intravenous administration at 3 h after IR), 6 (IR+EPCs/3.0cc/at 3h/24 h after IR), and euthanized by day 3 after IR. The left-ventricular-ejection-fraction, protein and cellular expressions of endothelial-cell markers (CD31/vWF), small vessel number and protein expression of mitochondrial (mitochondrial-cytochrome-C) integrity were highest in group 1, lowest in group 2, significantly higher in group 4 than in groups 3/5/6 and significantly higher in groups 3/6 than in group 5 but they showed no differences in groups3/6, whereas the protein expressions of apoptotic (cleaved-caspase 3/cleaved-PARP), fibrotic (Smad3/TGF-ß), mitochondrial-damaged (cytosolic-cytochrome-C), heart-failed/pressure-overload (BNP), oxidative-stress (p47phox/NOX-1/NOX-2/oxidized protein), and autophagic (LCB3-II/LCB3-I) biomarkers and fibrotic/collagen-deposition areas exhibited an opposite pattern to endothelial-cell markers (all P < .0001). The protein expressions of angiogenesis (VEGF/SDF-1α/CXCR4/HIF-1α) were lowest in group 1, highest in group 4, significantly higher in groups 3/6 than in groups 2/5, significantly higher in group 5 than in group 2, but they showed no difference between groups 3/6 (all P < .0001). These results demonstrate that two consecutive doses of EPC/CM were superior to just one at protecting LVM against IR injury.
机译：该研究测试了单剂量和两剂内皮祖细胞（EPC）和EPCS衍生的条件培养基（CM）免受急性缺血再灌注（IR）损伤的影响的冲击保护左心室心肌（LVM）。在体外研究表明EPC和CM与对照相比，EPCS和CM具有相当更高的血管生成的能力（所有P <.001）。成人 - 雄性SD大鼠（n = 36）同等分为1组（假手术控制），2（IR +载体），3 [IR + EPCS / 1.2×106 / IR手术后3小时的静脉内给药）， 4（IR + EPCS / 1.2×106 /在IR后的3 H / 24小时），5（IR + CM / 3.0CC / IR后3小时内给药），6（IR + EPCS / 3.0CC / 3小时/ IR后24小时），红外1天第3天安乐死。内皮细胞标记物（CD31 / VWF）的左心室射血分数，蛋白质和细胞表达，小血管数和线粒体（线粒体 - 细胞色素-C）完整性的小血管数和蛋白表达在第1组中最高，2组最低在第4组中显着高于3/5/6组，组3/6组显着高于第5组，但它们在组3/6中没有差异，而凋亡的蛋白质表达（切割 - caspase 3 /切割 - PARP），纤维化（SMAD3 / TGF-β），线粒体损伤（细胞溶质 - 细胞色素-C），心脏失效/压力过载（BNP），氧化应激（P47PHO / NOX-1 / NOX-2 /氧化蛋白）和自噬（LCB3-II / LCB3-I）生物标志物和纤维化/胶原沉积区域表现出对内皮细胞标记物的相反图案（所有P <.0001）。血管生成的蛋白表达（VEGF / SDF-1α/ CXCR4 / HIF-1α）在第1组中最低，第4组中最高，3/6组显着高于2/5组，比第5组显着高于第2组，但它们在3/6组之间没有差异（所有P <.0001）。这些结果表明，在保护LVM免受IR损伤的情况下，两次连续的两种剂量的EPC / CM均优于一种。

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