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首页> 美国卫生研究院文献>Communications Biology >Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
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Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

机译：基于光学干涉测量法的微量探剂吸气提供实时子纳米空间分辨率

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a The optical interrogator (Deltasens) is comprised of a superluminescent diode (SLD) connected to a depolarizer (DP) via a Panda fiber (pmf). The DP is then connected to a circulator (C) with a single mode fiber (smf), which reroutes the signal to the spectrometer and, via a 90/10 optical fiber splitter, to the sensors. b, c These are shown schematically (not to scale) to highlight the Fresnel reflections occurring at RI discontinuities (red arrows), with the resulting optical cavities (dimensions a to d). d Schematic cross-section of the probe where its components and the connection to the water reservoir and syringe pump are shown. During an experiment, a pressure variation at the probe nozzle can be operated either by changing the height of the water reservoir or by operating the syringe pump. e Fourier transform of an interference signal taken after capturing a 160 µm polystyrene bead (as shown in inset). The n subscripts refer to the RIs of the media between the interfaces, referring to the dimensioning shown in the sensors highlight (n* is a weighted average of nb and nc). Each peak contains phase information. By selectively tracking its variation over time, it is possible to obtain optical path length variation, δOPL, versus time plots. f Example of the unfiltered pressure sensor response to a DMA-like test. Note the sub-nanometer resolution, highlighted in the inset.
机译：光学询问器（Deltasens）由通过熊猫纤维（PMF）连接到Deporarizer（DP）的超发光二极管（SLD）组成。然后，DP通过单模光纤（SMF）连接到循环器（C），该光纤（SMF）将信号重新排出到光谱仪，并且通过90/10光纤分离器到传感器。 B，C示意性地示出（不缩放）以突出以R 1不连续（红色箭头）发生的菲涅耳反射，其中所得到的光学腔（尺寸A至D）。 D探头的示意性横截面，其中示出了其部件和与储水器和注射泵的连接。在实验期间，可以通过改变水库的高度或通过操作注射器泵来操作探针喷嘴处的压力变化。在捕获160μm聚苯乙烯珠（如插图中所示）之后取得干扰信号的傅立叶变换。 N下标是指接口之间的介质的RIS，参考传感器中所示的尺寸突出显示（N *是NB和NC的加权平均值）。每个峰值包含相位信息。通过选择性地跟踪其随时间的变化，可以获得光学路径长度变化，ΔO11与时间图。 F未过滤的压力传感器的例子响应DMA样测试。注意在插图中突出显示的子纳米分辨率。

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