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Species integrity introgression and genetic variation across a coral reef fish hybrid zone

机译:珊瑚礁鱼杂交区的物种完整性血栓增生和遗传变异

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摘要

Hybridization and introgression are evolutionarily significant phenomena breaking down species boundaries. “Hybrid zones” (regions of species overlap and hybridization) enable quantification of hybridization frequency and examination of mechanisms driving and maintaining gene flow. The hybrid anemonefish Amphiprion leucokranos is found where parent species (A. chrysopterus; A. sandaracinos) distributions overlap. Here, we examine geographic variation in hybridization and introgression, and potential impacts on parent species integrity through assessing relative abundance, social group composition, and genetic structure (mtDNA cytochrome b, 21 microsatellite loci) of taxa at three hybrid zone locations: Kimbe Bay (KB) and Kavieng (KA), Papua New Guinea; the Solomon Islands (SO). Relative abundances of and size disparities between parent species apparently drive hybridization frequency, introgression patterns, and genetic composition of taxa. Conspecific groups are most common in KB (65%) where parent species are similarly abundant. Conversely, mixed species groups dominate SO (82%), where A. chrysopterus is more abundant. Hybrids most commonly cohabit with A. sandaracinos in KB (17%), but with A. chrysopterus in KA (22%) and SO (50%). Genetic differentiation (nDNA) analyses indicate that parent species remain distinct, despite ongoing hybridization and hybrids are genetically similar to A. sandaracinos—resulting from persistent backcrossing with this smallest species. This study shows that hybridization outcomes may depend on the social and ecological context in which taxa hybridize, where relative abundance and disparate size of parent species explain the frequency and patterns of hybridization and introgression in the A. leucokranos hybrid zone, reflecting size‐based dominance behaviors of anemonefish social groups.
机译:杂交和迟发是进化的,分解了物种边界的显着现象。 “杂交区”(物种的区域重叠和杂交)使得杂交频率的定量能量和驱动和维持基因流动的机制检查。杂交anemonefish amphiprion leucokranos被发现在父片(A. chrysopterus; A. sandaracinos)分布重叠。在这里,我们研究杂交和迟发的地理变异,以及通过评估三个混合区位置的分类群的相对丰度,社会群体组成和遗传结构(MTDNA细胞色素B,21微卫星基因座)对母体物种完整性的潜在影响:Kimbe Bay( KB)和Kavieng(KA),巴布亚新几内亚;所罗门群岛(SO)。母体物种之间的相对丰富的和尺寸差异显然驱动杂交频率,血液模式和遗传构成的分类群。在母体物种相似丰富的KB(65%)中,本群的特异性群体最常见。相反,混合物种群体占主导地位(82%),其中A. Chrysopterus更丰富。杂交物最常与KB(17%)的桑达腊肠有同居队,但在Ka(22%)等中的蛹肠(50%)。遗传分化(NDNA)分析表明,尽管持续的杂交和杂交种仍然是不同的,但杂交种与A. SandaracinoS的持久性回复导致这种最小物种仍然是不同的。本研究表明,杂交结果可能取决于捕获毒率杂交的社会和生态背景,其中母体物种的相对丰度和不同大小解释了A. leucokranos杂交区的杂交和迟发的频率和模式,反映了基于尺寸的优势猩猩的行为社会群体。

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