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Trace Element Concentration and Stable Isotope Ratio Analysis in Blueberries and Bilberries: A Tool for Quality and Authenticity Control

机译：蓝莓和越桔中痕量元素浓度和稳定同位素比分析：一种质量和真实性控制的工具

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摘要

Vaccinium genus berries—wild bilberries (Vaccinium myrtillus L.) and cultivated highbush blueberries (Vaccinium corymbosum L.)—are consumed worldwide, and their consumption has a trend of stable increase. Thus, considering their wide use in ethnomedicine, for juice and jam production, as functional food, as well as their use in preparations of extracts which have application potential in pharmaceutical and cosmetics industries, studies regarding the composition of these berries are of special importance. The aim of this study is to characterise the elemental and isotopic composition, as well as variation in element concentration in bilberries gathered from different sites in Northern Europe and in commercially available blueberry samples from across the World. Furthermore, our aim was to develop tools for authenticity and quality control of these berries. The elemental composition of berries was analysed using inductively coupled plasma with optical emission detection (ICP-OED), while isotope ratio mass spectrometry (IRMS) was used for the determination of isotope ratio values. The results demonstrated detectable differences between macro- and microelement values in bilberries. IRMS analysis of blueberries revealed significant differences in isotope ratios based on the place of origin, indicating the possibility to use this analytical method for authenticity testing. In none of the samples, pollution was detected, even though there were indications of different growth conditions and geochemical differences affecting bilberry composition.
机译：丙酸瓦斯 - 野生百合（瓦米氏菌Myrtillus L.）和培养的高杂蓝莓（瓦西吡喃糖尿病） - 在全球范围内消费，他们的消费量趋势稳定增加。因此，考虑到它们在乙烯基因中广泛使用，用于果汁和果酱生产，作为功能性食品，以及它们在制药和化妆品行业的应用潜力的提取物的制剂中，关于这些浆果的组成的研究具有特殊重要性。本研究的目的是表征元素和同位素组合物，以及从欧洲北部的不同地点和来自世界各地的商业上可获得的蓝莓样品中的百叶菊中的元素浓度的变化。此外，我们的目标是开发这些浆果的真实性和质量控制的工具。使用具有光学发射检测（ICP-OED）的电感耦合等离子体分析浆果的元素组成，而同位素比质谱法（IRMS）用于测定同位素比值。结果表明越桔中的宏观和微元素值之间可检测到的差异。基于原产地，蓝莓的IRMS分析显示出同位素比率的显着差异，表明使用该分析方法进行真实性测试的可能性。在没有一个样品中，污染被发现，即使存在不同的生长条件和影响胆道组成的地球化学差异。

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