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Development of a 3D printed device to support long term intestinal culture as an alternative to hyperoxic chamber methods

机译:开发3D打印设备以支持长期肠道培养以替代高氧腔室方法

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摘要

BackgroundMost interactions between pathogenic microorganisms and their target host occur on mucosal surfaces of internal organs such as the intestine. In vitro organ culture (IVOC) provides an unique tool for studying host-pathogen interactions in a controlled environment. However, this technique requires a complex laboratory setup and specialized apparatus. In addition, issues arise when anaerobic pathogens are exposed to the hyperoxic environment required for intestinal culture. The objective of this study was to develop an accessible 3D–printed device that would allow manipulation of the gas mixture used to supply the tissue culture media separately from the gas mixture exposed to the mucosal side of explants.
机译:背景病原微生物与其目标宿主之间的大多数相互作用发生在内部器官(如肠道)的粘膜表面。体外器官培养(IVOC)提供了一种在受控环境中研究宿主与病原体相互作用的独特工具。但是,该技术需要复杂的实验室设置和专用设备。另外,当厌氧性病原体暴露于肠道培养所需的高氧环境时,也会出现问题。这项研究的目的是开发一种可访问的3D打印设备,该设备可以与用于暴露于外植体粘膜侧的气体混合物分开处理用于提供组织培养基的气体混合物。

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